RRC ID 72859
Author Tsurushima H, Liu SQ, Tsuboi K, Yoshii Y, Nose T, Ohno T.
Title Induction of human autologous cytotoxic T lymphocytes against minced tissues of glioblastoma multiforme.
Journal J Neurosurg
Abstract The authors induced autologous cytotoxic T lymphocytes (CTLs) directly from peripheral blood lymphocytes by preparing a coculture of minced tissue fragments of glioblastoma multiforme (GBM) with interleukins-1, -2, -4, and -6 and interferon-gamma in RHAM alpha medium containing 5% autologous plasma for 2 weeks. At the end of this period, the frequencies of CD3+, CD4+, CD8+, and CD16+ lymphocytes were 95% to 99%, 40% to 62%, 37% to 38%, and 0.2%, respectively. The lymphocytes killed 82% to 100% of the GBM cells within 48 hours at an effector-to-target cell ratio of 1.67, whereas in a separate coculture, autologous lymphokine-activated killer (LAK) cells killed only 33% of GBM cells under the same conditions. The lymphocytes showed no cytotoxicity against LAK-sensitive Daudi cells, natural killer-sensitive K562 cells or autologous fibroblasts grown from the brain tumor, although they did show slight cytotoxicities against allogeneic GBM cell lines. These results lead the authors to suggest that the lymphocyte population contains specific CTLs for autologous brain tumor cells and that these CTLs could be effective in adoptive immunotherapy to combat brain tumor.
Volume 84(2)
Pages 258-63
Published 1996-2-1
DOI 10.3171/jns.1996.84.2.0258
PMID 8592229
MeSH Adult Brain Neoplasms / immunology* Cells, Cultured Coculture Techniques Cytotoxicity, Immunologic Glioblastoma / immunology* Humans Interferon-gamma / pharmacology Interleukins / pharmacology Killer Cells, Lymphokine-Activated / immunology Male Monocytes / immunology T-Lymphocytes, Cytotoxic / immunology*
IF 3.968
Resource
Human and Animal Cells KG-1-C(RCB0270)