論文 - 詳細
| RRC ID | 72861 |
|---|---|
| 著者 | Ohno T, Wang X, Kurashima J, Saijo-Kurita K, Hirono M. |
| タイトル | A novel Vero cell line for use as a mammalian host-vector system in serum-free medium. |
| ジャーナル | Cytotechnology |
| Abstract |
We have established a novel cell line from a Vero cell derivative that is useful for expression of exogenous genes and protein production. Parental Vero-317 cells can grow in biotin-containing Eagle's MEM without supplements. By transforming this cell line with replication origin-defective SV40 DNA, which contains a temperature-sensitive tsA58 large T antigen gene, we established the Verots S3 cell line that amplified a SV40-origin containing plasmid. The cell line expressed a human growth hormone (hGH) gene insert with higher efficiency than COS-7 cells in 5% serum-containing MEM and could grow and continue hGH expression in protein-free MEM. However, temperature-sensitive shut down of hGH production was observed not immediately but 3 days after the temperature shift from 33 degrees C to 39.5 degrees C. |
| 巻・号 | 7(3) |
| ページ | 165-72 |
| 公開日 | 1991-11-1 |
| DOI | 10.1007/BF00365927 |
| PMID | 1368119 |
| MeSH | Animals Antigens, Polyomavirus Transforming / biosynthesis Blotting, Southern Cell Division Cell Line Culture Media, Serum-Free Gene Expression Genetic Vectors* Growth Hormone / genetics Growth Hormone / metabolism Kinetics Plasmids Restriction Mapping Temperature Transfection Transformation, Genetic Vero Cells / cytology Vero Cells / metabolism* |
| IF | 1.777 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |