| RRC ID |
72888
|
| 著者 |
Ogose A, Motoyama T, Hotta T, Watanabe H.
|
| タイトル |
Expression of bone morphogenetic proteins in human osteogenic and epithelial tumor cells.
|
| ジャーナル |
Pathol Int
|
| Abstract |
In addition to osteosarcomas some epithelial tumors also show bone induction, which is supposedly due to bone morphogenetic proteins (BMPs). However, the types of BMPs expressed in different kinds of tumors have not been studied extensively. In this study five human osteosarcoma and six human carcinoma cell lines were examined to investigate the bone induction and BMP mRNA expression. The cultured cells were transplanted in the subcutaneous tissue of athymic nude mice with or without diffusion chambers. Reverse transcription-polymerase chain reaction (RT-PCR) was used for the detection of BMP mRNAs. All osteosarcoma cell lines expressing all BMPs (BMP-1 to 7) examined, successfully formed bone or osteoid tissue irrespective of the use of a diffusion chamber. Bone formation was induced by diffusion chamber in an osteosarcoma cell line OST and a gastric adenocarcinoma cell line MKN45. OST cells lacked BMP-3 and 7, and MKN45 cells lacked BMP-3, 5 and 6. Our data suggest that BMP-3, 5, 6 and 7 are not always essential for bone induction in tumor cells.
|
| 巻・号 |
46(1)
|
| ページ |
9-14
|
| 公開日 |
1996-1-1
|
| DOI |
10.1111/j.1440-1827.1996.tb03527.x
|
| PMID |
10846544
|
| MeSH |
Adenocarcinoma / metabolism*
Adenocarcinoma / pathology
Animals
Bone Morphogenetic Proteins / biosynthesis*
Bone Morphogenetic Proteins / genetics
Bone Neoplasms / metabolism*
Bone Neoplasms / pathology
DNA Primers / chemistry
DNA, Neoplasm / analysis
Female
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Neoplasms, Experimental / metabolism*
Neoplasms, Experimental / pathology
Osteogenesis / drug effects
Osteosarcoma / metabolism*
Osteosarcoma / pathology
RNA, Messenger / metabolism
RNA, Neoplasm / analysis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
|
| IF |
2.11
|
| リソース情報 |
| ヒト・動物細胞 |
Saos-2(RCB0428)
OS-RC-2(RCB0735)
Hep G2(RCB0459) |
