| RRC ID |
72899
|
| Author |
Endoh H, Sasaki H, Maruyama K, Takeyama K, Waga I, Shimizu T, Kato S, Kawashima H.
|
| Title |
Rapid activation of MAP kinase by estrogen in the bone cell line.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We examined the effect of estrogen on mitogen-activated protein kinase (MAPK) in osteoblastic cells. Rat ROS 17/2.8 cells were exposed to 17beta-estradiol (E2) and MAPK activity in the cells was measured by an in vitro phosphorylation assay. E2 treatment caused a rapid and transient MAPK activation within 5 min. Insulin-like growth factor-I, which acts via their membrane receptors, caused a similar effect, but it required 10 min to reach the maximum level. Western blot analyses with anti-MAPK and anti-phosphotyrosine antibodies demonstrated that the E2 activation of MAPK was accompanied by phosphorylation of the enzyme. The concentration range (10 nM-1 pM) of E2 needed for this MAPK activation was less than that (1 microM-0.1 nM) needed for the transcriptional activation via the nuclear estrogen receptor (ER). These data provide the first evidence of MAPK activation by E2 through phosphorylation, which may be mediated through a putative plasma membrane receptor in the cultured bone cells.
|
| Volume |
235(1)
|
| Pages |
99-102
|
| Published |
1997-6-9
|
| DOI |
10.1006/bbrc.1997.6746
|
| PII |
S0006291X97967462
|
| PMID |
9196043
|
| MeSH |
Animals
Antibodies / immunology
Blotting, Western
Calcium-Calmodulin-Dependent Protein Kinases / immunology
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
Cell Line
Enzyme Activation
Estradiol / pharmacology*
Insulin-Like Growth Factor I / pharmacology
Osteoblasts / drug effects
Osteoblasts / enzymology*
Phosphorylation
Phosphotyrosine / immunology
Rats
Receptors, Estrogen / metabolism
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
ROS-17/2.8-5(RCB0462) |
