| RRC ID |
72909
|
| 著者 |
Shimokawa N, Sugama S, Miura M.
|
| タイトル |
Extracellular H+ stimulates the expression of c-fos/c-jun mRNA through Ca2+/calmodulin in PC12 cells.
|
| ジャーナル |
Cell Signal
|
| Abstract |
Evidence has accumulated that an increase in extracellular protons stimulates the transmembrane mechanism to induce various intracellular responses, such as the expression of c-fos and c-jun. In the present study, we aimed to obtain evidence that an increase in extracellular protons induces expression of c-fos/c-jun mRNA in PC12 pheochromocytoma cells of rats. We found that the c-fos/c-jun mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH values and the expression of c-fos/c-jun mRNA. To determine whether the Ca2+/calmodulin system subserves the H+-induced expression of c-fos/c-jun, Ca2+/calmodulin inhibitor trifluoperazine was added to PC12 cells. We found that trifluoperazine inhibited the expression of the H+-induced c-fos/c-jun mRNA by 30-35%. In contrast, trifluoperazine did not inhibit the expression of phorbol-induced c-fos/c-jun mRNA. These results indicate that an increase in extracellular protons induces the expression of c-fos/c-jun mRNA, and this expression is mediated partly by the Ca2+/calmodulin system.
|
| 巻・号 |
10(7)
|
| ページ |
499-503
|
| 公開日 |
1998-7-1
|
| DOI |
10.1016/s0898-6568(97)00176-9
|
| PII |
S0898-6568(97)00176-9
|
| PMID |
9754718
|
| MeSH |
Animals
Calcium / metabolism*
Calmodulin / metabolism*
Extracellular Space
Gene Expression Regulation*
Hydrogen*
PC12 Cells
Proto-Oncogene Proteins c-fos / genetics*
Proto-Oncogene Proteins c-jun / genetics*
Rats
|
| IF |
3.968
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
