RRC ID 72909
Author Shimokawa N, Sugama S, Miura M.
Title Extracellular H+ stimulates the expression of c-fos/c-jun mRNA through Ca2+/calmodulin in PC12 cells.
Journal Cell Signal
Abstract Evidence has accumulated that an increase in extracellular protons stimulates the transmembrane mechanism to induce various intracellular responses, such as the expression of c-fos and c-jun. In the present study, we aimed to obtain evidence that an increase in extracellular protons induces expression of c-fos/c-jun mRNA in PC12 pheochromocytoma cells of rats. We found that the c-fos/c-jun mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH values and the expression of c-fos/c-jun mRNA. To determine whether the Ca2+/calmodulin system subserves the H+-induced expression of c-fos/c-jun, Ca2+/calmodulin inhibitor trifluoperazine was added to PC12 cells. We found that trifluoperazine inhibited the expression of the H+-induced c-fos/c-jun mRNA by 30-35%. In contrast, trifluoperazine did not inhibit the expression of phorbol-induced c-fos/c-jun mRNA. These results indicate that an increase in extracellular protons induces the expression of c-fos/c-jun mRNA, and this expression is mediated partly by the Ca2+/calmodulin system.
Volume 10(7)
Pages 499-503
Published 1998-7-1
DOI 10.1016/s0898-6568(97)00176-9
PII S0898-6568(97)00176-9
PMID 9754718
MeSH Animals Calcium / metabolism* Calmodulin / metabolism* Extracellular Space Gene Expression Regulation* Hydrogen* PC12 Cells Proto-Oncogene Proteins c-fos / genetics* Proto-Oncogene Proteins c-jun / genetics* Rats
IF 3.968
Human and Animal Cells PC-12(RCB0009)