RRC ID 72934
著者 Wada T, Yoshikawa Y, Tokuyama S, Kuwabara M, Akita H, Miyagi T.
タイトル Cloning, expression, and chromosomal mapping of a human ganglioside sialidase.
ジャーナル Biochem Biophys Res Commun
Abstract Here we report the cDNA sequence of a human ganglioside sialidase. The cDNA was isolated from a human brain cDNA library by screening with a 240 bp probe generated by polymerase chain reaction using primers based on the sequences of rat cytosolic and bovine membrane sialidases which we previously cloned. The 3.0 kb cDNA encodes an open reading frame of 436 amino acids containing a putative transmenbrane domain and an Arg-Ile-Pro and three Asp-box sequences characteristic of sialidases and showing overall 83% and 39% identities to the bovine and rat enzymes, respectively. Northern blot analysis revealed high expression in skeletal muscle and testis, but low level in kidney, placenta, lung, and digestive organs. Transient expression of the cDNA in COS-1 cells resulted in a 130-fold increase in sialidase activity compared to the control level, and the activity was found to be almost specific for gangliosides. Fluorescent in situ hybridization allowed the human sialidase gene localized to chromosome 11 at q 13.5.
巻・号 261(1)
ページ 21-7
公開日 1999-7-22
DOI 10.1006/bbrc.1999.0973
PII S0006-291X(99)90973-7
PMID 10405317
MeSH Amino Acid Sequence Animals Base Sequence Blotting, Northern COS Cells Cattle Cell Membrane / enzymology Chromosomes, Human, Pair 11 / genetics Cloning, Molecular Gene Expression* Humans Hydrogen-Ion Concentration Molecular Sequence Data Neuraminidase / genetics* Neuraminidase / metabolism Physical Chromosome Mapping* Rats Sequence Homology, Amino Acid Substrate Specificity Transfection
IF 2.985
リソース情報
ヒト・動物細胞 COS-1(RCB143)