RRC ID 72940
Author Birot A, Kus K, Priest E, Al Alwash A, Castello A, Mohammed S, Vasiljeva L, Kilchert C.
Title RNA-binding protein Mub1 and the nuclear RNA exosome act to fine-tune environmental stress response.
Journal Life Sci Alliance
Abstract The nuclear RNA exosome plays a key role in controlling the levels of multiple protein-coding and non-coding RNAs. Recruitment of the exosome to specific RNA substrates is mediated by RNA-binding co-factors. The transient interaction between co-factors and the exosome as well as the rapid decay of RNA substrates make identification of exosome co-factors challenging. Here, we use comparative poly(A)+ RNA interactome capture in fission yeast expressing three different mutants of the exosome to identify proteins that interact with poly(A)+ RNA in an exosome-dependent manner. Our analyses identify multiple RNA-binding proteins whose association with RNA is altered in exosome mutants, including the zinc-finger protein Mub1. Mub1 is required to maintain the levels of a subset of exosome RNA substrates including mRNAs encoding for stress-responsive proteins. Removal of the zinc-finger domain leads to loss of RNA suppression under non-stressed conditions, altered expression of heat shock genes in response to stress, and reduced growth at elevated temperature. These findings highlight the importance of exosome-dependent mRNA degradation in buffering gene expression networks to mediate cellular adaptation to stress.
Volume 5(2)
Published 2022-2-1
DOI 10.26508/lsa.202101111
PII 5/2/e202101111
PMID 34848435
PMC PMC8645331
MeSH Exosome Multienzyme Ribonuclease Complex / metabolism* Gene Expression Regulation, Fungal Gene-Environment Interaction RNA, Messenger / genetics* RNA, Messenger / metabolism RNA, Nuclear / genetics* RNA, Nuclear / metabolism RNA-Binding Proteins / metabolism* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Stress, Physiological*
IF 2.622
Yeast FY9118