論文 - 詳細
| RRC ID | 72940 |
|---|---|
| 著者 | Birot A, Kus K, Priest E, Al Alwash A, Castello A, Mohammed S, Vasiljeva L, Kilchert C. |
| タイトル | RNA-binding protein Mub1 and the nuclear RNA exosome act to fine-tune environmental stress response. |
| ジャーナル | Life Sci Alliance |
| Abstract |
The nuclear RNA exosome plays a key role in controlling the levels of multiple protein-coding and non-coding RNAs. Recruitment of the exosome to specific RNA substrates is mediated by RNA-binding co-factors. The transient interaction between co-factors and the exosome as well as the rapid decay of RNA substrates make identification of exosome co-factors challenging. Here, we use comparative poly(A)+ RNA interactome capture in fission yeast expressing three different mutants of the exosome to identify proteins that interact with poly(A)+ RNA in an exosome-dependent manner. Our analyses identify multiple RNA-binding proteins whose association with RNA is altered in exosome mutants, including the zinc-finger protein Mub1. Mub1 is required to maintain the levels of a subset of exosome RNA substrates including mRNAs encoding for stress-responsive proteins. Removal of the zinc-finger domain leads to loss of RNA suppression under non-stressed conditions, altered expression of heat shock genes in response to stress, and reduced growth at elevated temperature. These findings highlight the importance of exosome-dependent mRNA degradation in buffering gene expression networks to mediate cellular adaptation to stress. |
| 巻・号 | 5(2) |
| 公開日 | 2022-2-1 |
| DOI | 10.26508/lsa.202101111 |
| PII | 5/2/e202101111 |
| PMID | 34848435 |
| PMC | PMC8645331 |
| MeSH | Exosome Multienzyme Ribonuclease Complex / metabolism* Gene Expression Regulation, Fungal Gene-Environment Interaction RNA, Messenger / genetics* RNA, Messenger / metabolism RNA, Nuclear / genetics* RNA, Nuclear / metabolism RNA-Binding Proteins / metabolism* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Stress, Physiological* |
| IF | 2.622 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY9118 |