RRC ID 72943
Author Yoshikawa M, Toyohara M, Ueda S, Shiroi A, Takeuchi H, Nishiyama T, Yamada T, Fukui H, Ishizaka S.
Title Glycyrrhizin inhibits TNF-induced, but not Fas-mediated, apoptosis in the human hepatoblastoma line HepG2.
Journal Biol Pharm Bull
Abstract To determine the transaminase-lowering action of glycyrrhizin (GL) immunologically, the effect of GL on tumor necrosis factor (TNF)-alpha- and Fas-mediated apoptosis was assessed using a human hepatoblastoma line, HepG2 cells. The HepG2 cells were resistant to TNF-alpha and anti-Fas antibody, but were rendered susceptible to TNF-alpha and anti-Fas antibody in the presence of actinomycin D (Act D), an inhibitor of RNA synthesis. The cytotoxicity induced by TNF-alpha/Act D or anti-Fas/Act D was accompanied by DNA fragmentation, indicating apoptotic death of HepG2 cells. GL partially prevented the apoptosis of HepG2 cells induced by TNF-alpha/Act D in a GL-dose dependent fashion. However, this protective effect of GL was not observed in the cytotoxicity of HepG2 caused by anti-Fas/Act D. Although the protection mechanism of GL, observed in a limited fashion against TNF-alpha-mediated apoptosis, is unclear, the present results provide an immunological explanation for the transaminase-lowering action of GL in the GL treatment of chronic liver diseases involving apoptotic hepatocyte death in their pathogenesis.
Volume 22(9)
Pages 951-5
Published 1999-9-1
DOI 10.1248/bpb.22.951
PMID 10513619
MeSH Anti-Infective Agents / pharmacology* Antibodies Apoptosis* Glycyrrhizic Acid / pharmacology* Hepatoblastoma Humans Tumor Cells, Cultured Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / physiology* fas Receptor / immunology fas Receptor / physiology*
IF 1.863
Human and Animal Cells Hep G2(RCB0459)