| RRC ID |
72945
|
| 著者 |
Kaneko A, Suzuki S, Hara M, Mori J, Kumagai M, Yajima H, Yamashita K, Kakizawa T, Yamazaki M, Takeda T, Miyamoto T, Ichikawa K, Hashizume K.
|
| タイトル |
1,25-Dihydroxyvitamin D3 suppresses the expression of the VCAM-1 receptor, VLA-4 in human leukemic HL-60 cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Very late antigen-4 (VLA-4) is the complex with alpha4 and beta1 integrins, which is the receptors to fibronectin and VCAM-1. We evaluate the effect of 1,25(OH)2D3 on the expression of VLA-4 in human leukemic HL-60, U937 cells and human melanoma A375 cells. Flow cytometric analysis demonstrate that the expression of alpha4 integrin is negatively regulated in the cell lines we studied. The expression of beta1 integrin is also decreased in HL-60 and U937 cells. The mRNA expression of alpha4 integrin is significantly decreased by the treatment with 1,25(OH)2D3, whereas 1,25(OH)2D3 does not alter the expression of beta1 mRNA. The adhesion assay demonstrate that the number of adherent cells treated with 1, 25(OH)2D3 is significantly lower than that untreated on VCAM-1-coated wells. Because VCAM-1 is highly expressed in the endothelial cells, it is possible that 1,25(OH)2D3 prevents the attachment of the cells from the endothelial cells in vivo.
|
| 巻・号 |
255(2)
|
| ページ |
371-6
|
| 公開日 |
1999-2-16
|
| DOI |
10.1006/bbrc.1999.0186
|
| PII |
S0006291X99901869
|
| PMID |
10049715
|
| MeSH |
Antigens, CD / biosynthesis
Antigens, CD / genetics
Calcitriol / pharmacology*
Cell Adhesion / drug effects
Cell Membrane / drug effects
Cell Membrane / metabolism
HL-60 Cells
Humans
Integrin alpha4
Integrin alpha4beta1
Integrin beta1 / biosynthesis
Integrin beta1 / genetics
Integrins / antagonists & inhibitors*
Integrins / biosynthesis
Integrins / genetics
Leukemia, Promyelocytic, Acute / metabolism*
Ligands
RNA, Messenger / antagonists & inhibitors
RNA, Messenger / biosynthesis
Receptors, Lymphocyte Homing / antagonists & inhibitors*
Receptors, Lymphocyte Homing / biosynthesis
Receptors, Lymphocyte Homing / genetics
Receptors, Very Late Antigen / antagonists & inhibitors*
Receptors, Very Late Antigen / biosynthesis
U937 Cells
Vascular Cell Adhesion Molecule-1 / metabolism*
Vascular Cell Adhesion Molecule-1 / physiology
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB0041)
U-937 DE-4(RCB0435) |
