RRC ID 72993
Author Kim CH, Todoroki T, Matsumura M, Ohno T.
Title Eligibility of antigenic-peptide-pre-loaded and fixed adhesive peripheral blood cells for induction of cytotoxic T lymphocytes from cancer patients with elevated serum levels of carcinoembryonic antigen.
Journal J Cancer Res Clin Oncol
Abstract The inducibility of cytotoxic T lymphocytes (CTL) that react with carcinoembryonic antigen (CEA) was tested in cancer patients with elevated (more than 5 ng/ml) serum CEA levels when antigen presentation was carried out with paraformaldehyde-fixed adhesive peripheral blood mononuclear cells (PBMC) from the patient that had been pre-loaded with CEA652(9), an HLA-A2402-restricted tumor antigenic peptide derived from CEA. By culturing fresh autologous PBMC on the fixed cell layer in medium containing interleukin-1, -2, -4 and -6. three out of eight patients developed CTL. The CTL from two of these patients killed CEA-protein-producing gastric cancer cells carrying HLA-A2402 and the cells from the remaining patient killed CEA-non-producing stomach cancer cells pre-loaded with CEA652(9). The results suggest that a single antigenic peptide on the fixed adhesive cells will allow the ex vivo induction of peptide-reactive CTL that are easier to handle and allow antigen presentation without tedious preculture of the "professional" antigen-presenting dendritic-cells.
Volume 126(7)
Pages 383-90
Published 2000-7-1
DOI 10.1007/pl00008486
PMID 10929760
MeSH Adult Aged Antibodies, Monoclonal Antigen Presentation Carcinoembryonic Antigen / blood Carcinoembryonic Antigen / immunology* Carcinoma / immunology* Cells, Cultured Cytokines / biosynthesis Cytotoxicity, Immunologic Dendritic Cells / immunology Epitopes, T-Lymphocyte / immunology Flow Cytometry HLA-A Antigens / immunology HLA-A24 Antigen Humans Immunophenotyping Leukocytes, Mononuclear / immunology Middle Aged T-Lymphocytes, Cytotoxic / immunology* Tumor Cells, Cultured
IF 3.656
Human and Animal Cells GT3TKB(RCB0885) MKN45(RCB1001)