| RRC ID |
72993
|
| Author |
Kim CH, Todoroki T, Matsumura M, Ohno T.
|
| Title |
Eligibility of antigenic-peptide-pre-loaded and fixed adhesive peripheral blood cells for induction of cytotoxic T lymphocytes from cancer patients with elevated serum levels of carcinoembryonic antigen.
|
| Journal |
J Cancer Res Clin Oncol
|
| Abstract |
The inducibility of cytotoxic T lymphocytes (CTL) that react with carcinoembryonic antigen (CEA) was tested in cancer patients with elevated (more than 5 ng/ml) serum CEA levels when antigen presentation was carried out with paraformaldehyde-fixed adhesive peripheral blood mononuclear cells (PBMC) from the patient that had been pre-loaded with CEA652(9), an HLA-A2402-restricted tumor antigenic peptide derived from CEA. By culturing fresh autologous PBMC on the fixed cell layer in medium containing interleukin-1, -2, -4 and -6. three out of eight patients developed CTL. The CTL from two of these patients killed CEA-protein-producing gastric cancer cells carrying HLA-A2402 and the cells from the remaining patient killed CEA-non-producing stomach cancer cells pre-loaded with CEA652(9). The results suggest that a single antigenic peptide on the fixed adhesive cells will allow the ex vivo induction of peptide-reactive CTL that are easier to handle and allow antigen presentation without tedious preculture of the "professional" antigen-presenting dendritic-cells.
|
| Volume |
126(7)
|
| Pages |
383-90
|
| Published |
2000-7-1
|
| DOI |
10.1007/pl00008486
|
| PMID |
10929760
|
| MeSH |
Adult
Aged
Antibodies, Monoclonal
Antigen Presentation
Carcinoembryonic Antigen / blood
Carcinoembryonic Antigen / immunology*
Carcinoma / immunology*
Cells, Cultured
Cytokines / biosynthesis
Cytotoxicity, Immunologic
Dendritic Cells / immunology
Epitopes, T-Lymphocyte / immunology
Flow Cytometry
HLA-A Antigens / immunology
HLA-A24 Antigen
Humans
Immunophenotyping
Leukocytes, Mononuclear / immunology
Middle Aged
T-Lymphocytes, Cytotoxic / immunology*
Tumor Cells, Cultured
|
| IF |
3.656
|
| Resource |
| Human and Animal Cells |
GT3TKB(RCB0885)
MKN45(RCB1001) |
