論文 - 詳細
| RRC ID | 73001 |
|---|---|
| 著者 | Kotani N, Nakano T, Kuwahara R. |
| タイトル | Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis. |
| ジャーナル | J Biol Chem |
| Abstract |
Coronavirus disease represents a real threat to the global population, and understanding the biological features of the causative virus, that is, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is imperative for mitigating this threat. Analyses of proteins such as primary receptors and coreceptors (cofactors), which are involved in the entry of SARS-CoV-2 into host cells, will provide important clues to help control the virus. Here, we identified host cell membrane protein candidates present in proximity to the attachment sites of SARS-CoV-2 spike proteins, using proximity labeling and proteomic analysis. The identified proteins represent key candidate factors that may be required for viral entry. We found SARS-CoV-2 host protein DPP4, cell adhesion protein Cadherin 17, and glycoprotein CD133 colocalized with cell membrane-bound SARS-CoV-2 spike proteins in Caco-2 cells and thus showed potential as candidate factors. Additionally, our analysis of the experimental infection of HEK293T cells with a SARS-CoV-2 pseudovirus indicated a 2-fold enhanced infectivity in the CD133-ACE2-coexpressing HEK293T cells compared to that in HEK293T cells expressing ACE-2 alone. The information and resources regarding these coreceptor labeling and analysis techniques could be utilized for the development of antiviral agents against SARS-CoV-2 and other emerging viruses. |
| 巻・号 | 298(11) |
| ページ | 102500 |
| 公開日 | 2022-11-1 |
| DOI | 10.1016/j.jbc.2022.102500 |
| PII | S0021-9258(22)00943-7 |
| PMID | 36152751 |
| PMC | PMC9492400 |
| MeSH | Angiotensin-Converting Enzyme 2 COVID-19* Caco-2 Cells HEK293 Cells Humans Membrane Proteins* / metabolism Protein Binding Proteomics Receptors, Virus / metabolism SARS-CoV-2 Spike Glycoprotein, Coronavirus* / metabolism Virus Attachment* Virus Internalization |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) A549(RCB0098) |