RRC ID 73001
著者 Kotani N, Nakano T, Kuwahara R.
タイトル Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis.
ジャーナル J Biol Chem
Abstract Coronavirus disease represents a real threat to the global population, and understanding the biological features of the causative virus, that is, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is imperative for mitigating this threat. Analyses of proteins such as primary receptors and coreceptors (cofactors), which are involved in the entry of SARS-CoV-2 into host cells, will provide important clues to help control the virus. Here, we identified host cell membrane protein candidates present in proximity to the attachment sites of SARS-CoV-2 spike proteins, using proximity labeling and proteomic analysis. The identified proteins represent key candidate factors that may be required for viral entry. We found SARS-CoV-2 host protein DPP4, cell adhesion protein Cadherin 17, and glycoprotein CD133 colocalized with cell membrane-bound SARS-CoV-2 spike proteins in Caco-2 cells and thus showed potential as candidate factors. Additionally, our analysis of the experimental infection of HEK293T cells with a SARS-CoV-2 pseudovirus indicated a 2-fold enhanced infectivity in the CD133-ACE2-coexpressing HEK293T cells compared to that in HEK293T cells expressing ACE-2 alone. The information and resources regarding these coreceptor labeling and analysis techniques could be utilized for the development of antiviral agents against SARS-CoV-2 and other emerging viruses.
巻・号 298(11)
ページ 102500
公開日 2022-11-1
DOI 10.1016/j.jbc.2022.102500
PII S0021-9258(22)00943-7
PMID 36152751
PMC PMC9492400
MeSH Angiotensin-Converting Enzyme 2 COVID-19* Caco-2 Cells HEK293 Cells Humans Membrane Proteins* / metabolism Protein Binding Proteomics Receptors, Virus / metabolism SARS-CoV-2 Spike Glycoprotein, Coronavirus* / metabolism Virus Attachment* Virus Internalization
IF 4.238
リソース情報
ヒト・動物細胞 293T(RCB2202) A549(RCB0098)