RRC ID 73037
著者 Wakabayashi I, Yasui K, Negoro M.
タイトル Diverse effects of ethanol on the pathway of inducible prostaglandin E2 production in macrophages.
ジャーナル Prostaglandins Other Lipid Mediat
Abstract The effects of ethanol on inducible prostaglandin production in RAW macrophages were investigated. Indomethacin (1 microM) or cycloheximide (1 microM) abolished prostaglandin E2 (PGE2) production induced by lipopolysaccharide (LPS, 1 microg/ml). Ethanol at concentrations from 100 mM to 600 mM concentration-dependently inhibited inducible PGE2 production, while ethanol only at higher concentrations (400 mM or more) showed cytotoxity to the cells. Cyclooxygenase-2 (COX-2) activity, estimated by transformation of exogenous arachidonic acid into PGE2, was not affected by ethanol (100-400 mM). LPS-induced expression of COX-2 mRNA was inhibited by ethanol (50-400 mM). On the other hand, protein expression of COX-2 by LPS was significantly increased by ethanol (100-400 mM). Ethanol alone at concentrations up to 600 mM did not induce expression of COX-2 protein. In a medium containing arachidonic acid (1 microM), ethanol at a low concentration (100 mM) did not significantly affect LPS-induced PGE2 production. These results suggest that ethanol shows diverse effects on the pathway of inducible PGE2 production in macrophages. Finally, ethanol may suppress utilization of arachidonic acid, resulting in reduction of inducible PGE2 production. Further study is needed to elucidate the mechanism of dissociation of ethanol effects on protein and mRNA expression.
巻・号 67(1)
ページ 61-74
公開日 2002-1-1
DOI 10.1016/s0090-6980(01)00176-9
PII S0090-6980(01)00176-9
PMID 11789898
MeSH Animals Cells, Cultured Culture Media / chemistry Cyclooxygenase 2 Dinoprostone / metabolism* Ethanol / pharmacology* Isoenzymes / drug effects Isoenzymes / genetics Isoenzymes / metabolism L-Lactate Dehydrogenase / drug effects L-Lactate Dehydrogenase / metabolism Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / metabolism* Mice Prostaglandin-Endoperoxide Synthases / drug effects Prostaglandin-Endoperoxide Synthases / genetics Prostaglandin-Endoperoxide Synthases / metabolism RNA, Messenger / drug effects
IF 2.283
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)