RRC ID 73057
著者 Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K.
タイトル Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species.
ジャーナル J Biosci Bioeng
Abstract The N- and O-linked oligosaccharides from fission yeast Schizosaccharomyces pombe not only contain large amounts of d-mannose (Man) but also contain large amounts of d-galactose (Gal). Although the galactomannans of S. pombe are mainly composed of α1,2- or α1,3-linked Gals, some of the terminal α1,2-linked Gals are found to be linked to pyruvylated β1,3-linked galactose (PvGal). We have determined the structural characteristics of the N-glycans and O-glycans in three Schizosaccharomyces species (S. japonicus, S. octosporus, and S. cryophilus) using lectin blot, 1H NMR spectroscopy, and size-fractionation high performance liquid chromatography (HPLC), and found that the galactosylation of oligosaccharides was a common feature in fission yeasts. In addition, each of the terminal Galα1,2-, Galβ1,3- and non-substituted Man residues exhibited distinct characteristics. A BLAST search of gene databases in Schizosaccharomyces identified genes homologous to pvg1 encoding pyruvyltransferase of S. pombe. These genes, when expressed in an S. pombe pvg1Δ strains, led to the pyruvylation of non-reducing terminal β-linked Gal, suggesting the biosynthetic pathway of PvGal-containing oligosaccharides is highly conserved in fission yeasts.
巻・号 130(2)
ページ 128-136
公開日 2020-8-1
DOI 10.1016/j.jbiosc.2020.03.008
PII S1389-1723(20)30172-9
PMID 32265131
MeSH Lectins / chemistry Magnetic Resonance Spectroscopy Molecular Structure Oligosaccharides / chemistry* Oligosaccharides / metabolism Polysaccharides / chemistry* Schizosaccharomyces / chemistry* Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics
IF 2.366
リソース情報
酵母 ARC039 (h-ura4-C190T leu1-32), FY16936, FY16937, FY21620