RRC ID 73071
Author Liu W, Zheng F, Wang Y, Fu C.
Title Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope.
Journal J Mol Cell Biol
Abstract Microtubules grow not only from the centrosome but also from various noncentrosomal microtubule-organizing centers (MTOCs), including the nuclear envelope (NE) and pre-existing microtubules. The evolutionarily conserved proteins Mto1/CDK5RAP2 and Alp14/TOG/XMAP215 have been shown to be involved in promoting microtubule nucleation. However, it has remained elusive as to how the microtubule nucleation promoting factors are specified to various noncentrosomal MTOCs, particularly the NE, and how these proteins coordinate to organize microtubule assembly. Here, we demonstrate that in the fission yeast Schizosaccharomyces pombe, efficient interphase microtubule growth from the NE requires Alp7/TACC, Alp14/TOG/XMAP215, and Mto1/CDK5RAP2. The absence of Alp7, Alp14, or Mto1 compromises microtubule regrowth on the NE in cells undergoing microtubule repolymerization. We further demonstrate that Alp7 and Mto1 interdependently localize to the NE in cells without microtubules and that Alp14 localizes to the NE in an Alp7 and Mto1-dependent manner. Tethering Mto1 to the NE in cells lacking Alp7 partially restores microtubule number and the efficiency of microtubule generation from the NE. Hence, our study delineates that Alp7, Alp14, and Mto1 work in concert to regulate interphase microtubule regrowth on the NE.
Volume 11(11)
Pages 944-955
Published 2019-12-23
DOI 10.1093/jmcb/mjz038
PII 5489040
PMID 31087092
PMC PMC6927237
MeSH Carrier Proteins / metabolism* Interphase / physiology* Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Models, Biological Nuclear Envelope / metabolism* Protein Binding Protein Transport Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / metabolism*
Resource
Yeast