論文 - 詳細
| RRC ID | 73104 |
|---|---|
| 著者 | Matsumura T, Totani H, Gunji Y, Fukuda M, Yokomori R, Deng J, Rethnam M, Yang C, Tan TK, Karasawa T, Kario K, Takahashi M, Osato M, Sanda T, Suda T. |
| タイトル | A Myb enhancer-guided analysis of basophil and mast cell differentiation. |
| ジャーナル | Nat Commun |
| Abstract |
The transcription factor MYB is a crucial regulator of hematopoietic stem and progenitor cells. However, the nature of lineage-specific enhancer usage of the Myb gene is largely unknown. We identify the Myb -68 enhancer, a regulatory element which marks basophils and mast cells. Using the Myb -68 enhancer activity, we show a population of granulocyte-macrophage progenitors with higher potential to differentiate into basophils and mast cells. Single cell RNA-seq demonstrates the differentiation trajectory is continuous from progenitors to mature basophils in vivo, characterizes bone marrow cells with a gene signature of mast cells, and identifies LILRB4 as a surface marker of basophil maturation. Together, our study leads to a better understanding of how MYB expression is regulated in a lineage-associated manner, and also shows how a combination of lineage-related reporter mice and single-cell transcriptomics can overcome the rarity of target cells and enhance our understanding of gene expression programs that control cell differentiation in vivo. |
| 巻・号 | 13(1) |
| ページ | 7064 |
| 公開日 | 2022-11-18 |
| DOI | 10.1038/s41467-022-34906-1 |
| PII | 10.1038/s41467-022-34906-1 |
| PMID | 36400777 |
| PMC | PMC9674656 |
| MeSH | Animals Basophils* Cell Differentiation / genetics Hematopoiesis* Leukocyte Count Mice Stem Cells / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | P-815(RCB1167) |