論文 - 詳細
| RRC ID | 73106 |
|---|---|
| 著者 | Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B. |
| タイトル | A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion. |
| ジャーナル | J Cell Biol |
| Abstract |
Mammalian sperm-egg adhesion depends on the trans-interaction between the sperm-specific type I glycoprotein IZUMO1 and its oocyte-specific GPI-anchored receptor JUNO. However, the mechanisms and proteins (fusogens) that mediate the following step of gamete fusion remain unknown. Using live imaging and content mixing assays in a heterologous system and structure-guided mutagenesis, we unveil an unexpected function for IZUMO1 in cell-to-cell fusion. We show that IZUMO1 alone is sufficient to induce fusion, and that this ability is retained in a mutant unable to bind JUNO. On the other hand, a triple mutation in exposed aromatic residues prevents this fusogenic activity without impairing JUNO interaction. Our findings suggest a second function for IZUMO1 as a unilateral mouse gamete fusogen. |
| 巻・号 | 222(2) |
| 公開日 | 2023-2-6 |
| DOI | 10.1083/jcb.202207147 |
| PII | 213693 |
| PMID | 36394541 |
| PMC | PMC9671554 |
| MeSH | Animals Cell Fusion Immunoglobulins* / metabolism Male Membrane Proteins* / metabolism Mice Receptors, Cell Surface* / metabolism Semen / metabolism Sperm-Ovum Interactions* Spermatozoa / metabolism |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 71 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.0 |
| リソース情報 | |
| ヒト・動物細胞 | BHK-21(RCB1423) |