RRC ID 73106
著者 Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B.
タイトル A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
ジャーナル J Cell Biol
Abstract Mammalian sperm-egg adhesion depends on the trans-interaction between the sperm-specific type I glycoprotein IZUMO1 and its oocyte-specific GPI-anchored receptor JUNO. However, the mechanisms and proteins (fusogens) that mediate the following step of gamete fusion remain unknown. Using live imaging and content mixing assays in a heterologous system and structure-guided mutagenesis, we unveil an unexpected function for IZUMO1 in cell-to-cell fusion. We show that IZUMO1 alone is sufficient to induce fusion, and that this ability is retained in a mutant unable to bind JUNO. On the other hand, a triple mutation in exposed aromatic residues prevents this fusogenic activity without impairing JUNO interaction. Our findings suggest a second function for IZUMO1 as a unilateral mouse gamete fusogen.
巻・号 222(2)
公開日 2023-2-6
DOI 10.1083/jcb.202207147
PII 213693
PMID 36394541
PMC PMC9671554
MeSH Animals Cell Fusion Immunoglobulins* / metabolism Male Membrane Proteins* / metabolism Mice Receptors, Cell Surface* / metabolism Semen / metabolism Sperm-Ovum Interactions* Spermatozoa / metabolism
IF 8.811
リソース情報
ヒト・動物細胞 BHK-21(RCB1423)