RRC ID 73108
Author Kuga T, Inoue N, Sometani K, Murataka S, Saraya M, Sugita R, Mikami T, Takeda Y, Taniguchi M, Nishida K, Yamagishi N.
Title The conserved C-terminal residues of FAM83H are required for the recruitment of casein kinase 1 to the keratin cytoskeleton.
Journal Sci Rep
Abstract The casein kinase 1 (CK1) family of serine/threonine protein kinases is involved in diverse cellular events at discrete subcellular compartments. FAM83H acts as a scaffold protein that recruits CK1 to the keratin cytoskeleton or to the nuclear speckles, which are storage sites for splicing factors. We determined the amino acid region of FAM83H required for recruiting CK1 to the keratin cytoskeleton. The subcellular localization of mutant FAM83H proteins with deletions of amino acid residues at different positions was evaluated via immunofluorescence. FAM83H mutants with deleted C-terminal residues 1134-1139, which are conserved among vertebrates, lost the ability to localize and recruit CK1 to the keratin cytoskeleton, suggesting that these residues are required for recruiting CK1 to the keratin cytoskeleton. The deletion of these residues (1134-1139) translocated FAM83H and CK1 to the nuclear speckles. Amino acid residues 1 to 603 of FAM83H were determined to contain the region responsible for the recruitment of CK1 to the nuclear speckles. Our results indicated that FAM83H recruits CK1 preferentially to the keratin cytoskeleton and alternatively to the nuclear speckles.
Volume 12(1)
Pages 11819
Published 2022-7-12
DOI 10.1038/s41598-022-16153-y
PII 10.1038/s41598-022-16153-y
PMID 35821396
PMC PMC9276658
MeSH Amino Acids / metabolism Animals Casein Kinase I* / genetics Casein Kinase I* / metabolism Casein Kinases / metabolism Cytoskeleton / metabolism Keratins* / genetics Keratins* / metabolism Microtubules / metabolism Mutant Proteins / metabolism
IF 3.998
Resource
DNA material Genome Network Project Human cDNA IRAL005B16 (HGY082040)