論文 - 詳細
| RRC ID | 73108 |
|---|---|
| 著者 | Kuga T, Inoue N, Sometani K, Murataka S, Saraya M, Sugita R, Mikami T, Takeda Y, Taniguchi M, Nishida K, Yamagishi N. |
| タイトル | The conserved C-terminal residues of FAM83H are required for the recruitment of casein kinase 1 to the keratin cytoskeleton. |
| ジャーナル | Sci Rep |
| Abstract |
The casein kinase 1 (CK1) family of serine/threonine protein kinases is involved in diverse cellular events at discrete subcellular compartments. FAM83H acts as a scaffold protein that recruits CK1 to the keratin cytoskeleton or to the nuclear speckles, which are storage sites for splicing factors. We determined the amino acid region of FAM83H required for recruiting CK1 to the keratin cytoskeleton. The subcellular localization of mutant FAM83H proteins with deletions of amino acid residues at different positions was evaluated via immunofluorescence. FAM83H mutants with deleted C-terminal residues 1134-1139, which are conserved among vertebrates, lost the ability to localize and recruit CK1 to the keratin cytoskeleton, suggesting that these residues are required for recruiting CK1 to the keratin cytoskeleton. The deletion of these residues (1134-1139) translocated FAM83H and CK1 to the nuclear speckles. Amino acid residues 1 to 603 of FAM83H were determined to contain the region responsible for the recruitment of CK1 to the nuclear speckles. Our results indicated that FAM83H recruits CK1 preferentially to the keratin cytoskeleton and alternatively to the nuclear speckles. |
| 巻・号 | 12(1) |
| ページ | 11819 |
| 公開日 | 2022-7-12 |
| DOI | 10.1038/s41598-022-16153-y |
| PII | 10.1038/s41598-022-16153-y |
| PMID | 35821396 |
| PMC | PMC9276658 |
| MeSH | Amino Acids / metabolism Animals Casein Kinase I* / genetics Casein Kinase I* / metabolism Casein Kinases / metabolism Cytoskeleton / metabolism Keratins* / genetics Keratins* / metabolism Microtubules / metabolism Mutant Proteins / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | p3xFLAG-CMV14-FAM83H (RDB20248) Genome Network Project Human cDNA IRAL005B16 (HGY082040) |