Reference - Detail
| RRC ID | 73120 |
|---|---|
| Author | Ishida-Ishihara S, Takada R, Furusawa K, Ishihara S, Haga H. |
| Title | Improvement of the cell viability of hepatocytes cultured in three-dimensional collagen gels using pump-free perfusion driven by water level difference. |
| Journal | Sci Rep |
| Abstract |
Cell-containing collagen gels are one of the materials employed in tissue engineering and drug testing. A collagen gel is a useful three-dimensional (3D) scaffold that improves various cell functions compared to traditional two-dimensional plastic substrates. However, owing to poor nutrient availability, cells are not viable in thick collagen gels. Perfusion is an effective method for supplying nutrients to the gel. In this study, we maintained hepatocytes embedded in a 3D collagen gel using a simple pump-free perfusion cell culture system with ordinary cell culture products. Flow was generated by the difference in water level in the culture medium. Hepatocytes were found to be viable in a collagen gel of thickness 3.26 (± 0.16 S.E.)-mm for 3 days. In addition, hepatocytes had improved proliferation and gene expression related to liver function in a 3D collagen gel compared to a 2D culture dish. These findings indicate that our perfusion method is useful for investigating the cellular functions of 3D hydrogels. |
| Volume | 12(1) |
| Pages | 20269 |
| Published | 2022-11-24 |
| DOI | 10.1038/s41598-022-24423-y |
| PII | 10.1038/s41598-022-24423-y |
| PMID | 36434099 |
| PMC | PMC9700666 |
| MeSH | Cell Survival Cells, Cultured Collagen / metabolism Gels / metabolism Hepatocytes* / metabolism Perfusion Water* / metabolism |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2(RCB1886) |