| RRC ID |
73138
|
| 著者 |
Takebayashi-Suzuki K, Uchida M, Suzuki A.
|
| タイトル |
Zbtb21 is required for the anterior-posterior patterning of neural tissue in the early Xenopus embryo.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The vertebrate body is organized along the dorsal-ventral (DV) and anterior-posterior (AP) axes by the BMP and Wnt pathways, respectively. We previously reported that Xenopus Zbtb14 promotes dorsalization (neural induction) of ectoderm by inhibiting BMP signaling and also posteriorizes neural tissue by activating Wnt signaling, thereby coordinating the patterning of the DV and AP axes during early development. Although it has been reported that human ZBTB21 binds to ZBTB14 and is involved in gene expression in cultured mammalian cells, the function of Zbtb21 in early embryonic development remains unknown. Here, we show that Xenopus Zbtb21 plays an essential role in AP axis formation in the early Xenopus embryo. zbtb21 and zbtb14 are co-expressed in the dorsal region of embryos during gastrulation. Simultaneous overexpression of Zbtb21 and Zbtb14 in ectodermal explants enhances the neural-inducing activity of Zbtb14. Moreover, knockdown experiments showed that Zbtb21 is required for the formation of posterior neural tissue and the suppression of anterior neural development. Collectively, these results suggest that in cooperation with Zbtb14, Zbtb21 has a crucial function in AP patterning during early Xenopus embryogenesis.
|
| 巻・号 |
630
|
| ページ |
190-197
|
| 公開日 |
2022-11-19
|
| DOI |
10.1016/j.bbrc.2022.09.048
|
| PII |
S0006-291X(22)01304-3
|
| PMID |
36166855
|
| MeSH |
Animals
Body Patterning* / genetics
Ectoderm
Embryo, Nonmammalian
Gene Expression Regulation, Developmental*
Wnt Signaling Pathway
Xenopus Proteins* / genetics
Xenopus Proteins* / metabolism
Xenopus laevis
|
| IF |
2.985
|
| リソース情報 |
| ツメガエル・イモリ |
Xenopus tropicalis |
