論文 - 詳細
| RRC ID | 73170 |
|---|---|
| 著者 | Kikuchi H, Harata K, Akiyoshi S, Sagara T, Madhyastha H, Kuribayashi F. |
| タイトル | Potential role of green tea amino acid l-theanine in the activation of innate immune response by enhancing expression of cytochrome b558 responsible for the reactive oxygen species-generating ability of leukocytes. |
| ジャーナル | Microbiol Immunol |
| Abstract |
l-Theanine (N-ethyl- l-glutamine) is an analog of l-glutamine and l-glutamic acid, accounts for up to 50% of all free amino acids in green tea, and elicits an umami taste. As l-theanine also shows various physiological activities including immune response-modifying activities, it is expected to be an excellent health-promoting phytochemical agent. To know the influences of l-theanine on the human innate immune response, we investigated the effect of l-theanine on the superoxide anion (O2- )-generating system of leukocytes using U937 cells. The O2- -generating system in leukocytes consists of membrane cytochrome b558 protein (a complex of p22-phox and gp91-phox proteins) and cytosolic p40-phox, p47-phox, and p67-phox proteins. Addition of 500 μM l-theanine caused remarkable enhancement of the all-trans retinoic acid (ATRA)-induced O2- -generating activity (to ~470% of ATRA-treated cells), but not l-glutamine and l-glutamic acid. Semiquantitative RT-PCR showed that the transcription level of gp91-phox is significantly increased in ATRA and l-theanine-co-treated cells. Chromatin immunoprecipitation revealed that l-theanine enhances acetylations of Lys-9 and Lys-14 residues of histone H3 within the chromatin surrounding the promoter region of the gp91-phox gene. Immunoblotting demonstrated that membrane cytochrome b558 proteins remarkably accumulate in ATRA + l-theanine-treated cells. These results suggested that l-theanine brings about a remarkable accumulation of cytochrome b558 protein via upregulating the transcription of the gp91-phox gene during leukocyte differentiation, resulting in marked augmentation of the O2- -generating ability, which is one of the most important functions of leukocytes responsible for the innate immune system. |
| 巻・号 | 66(6) |
| ページ | 342-349 |
| 公開日 | 2022-6-1 |
| DOI | 10.1111/1348-0421.12977 |
| PMID | 35338668 |
| MeSH | Amino Acids Cytochromes b* Glutamates Glutamic Acid Glutamine / pharmacology Humans Immunity, Innate Leukocytes NADPH Oxidases* / genetics Neutrophils / metabolism Phosphoproteins / metabolism Reactive Oxygen Species Superoxides / metabolism Tea Tretinoin |
| IF | 1.566 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-937 DE-4(RCB0435) |