RRC ID 73187
Author Chatelain P, Blanchard C, Astier J, Klinguer A, Wendehenne D, Jeandroz S, Rosnoblet C.
Title Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens.
Journal Sci Rep
Abstract Microalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to the colonization of terrestrial life. The tolerance to different stresses is manifested by changes in gene expression, which can be monitored by quantifying the amounts of transcripts by RT-qPCR. The identification of optimal reference genes for experiment normalization was therefore necessary. In this study, using four statistical algorithms followed by the RankAggreg package, we determined the best reference gene pairs suitable for normalizing RT-qPCR data in K. nitens in response to three abiotic stresses: high salinity, PEG-induced dehydration and heat shock. Based on these reference genes, we were able to identify marker genes in response to the three abiotic stresses in K. nitens.
Volume 12(1)
Pages 18988
Published 2022-11-8
DOI 10.1038/s41598-022-23783-9
PII 10.1038/s41598-022-23783-9
PMID 36348043
PMC PMC9643330
MeSH Gene Expression Profiling Gene Expression Regulation, Plant* Genes, Plant Real-Time Polymerase Chain Reaction Reference Standards Salinity Streptophyta* / genetics Stress, Physiological / genetics
IF 3.998
Algae NIES-2285