RRC ID 73191
Author Islam S, Hassan F, Mu MM, Ito H, Koide N, Mori I, Yoshida T, Yokochi T.
Title Piceatannol prevents lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor (NF)-kappaB activation by inhibiting IkappaB kinase (IKK).
Journal Microbiol Immunol
Abstract The effect of piceatannol on lipopolysaccharide (LPS)-induced nitric oxide (NO) production was examined. Piceatannol significantly inhibited NO production in LPS-stimulated RAW 264.7 cells. The inhibition was due to the reduced expression of an inducible isoform of NO synthase (iNOS). The inhibitory effect of piceatannol was mediated by down-regulation of LPS-induced nuclear factor (NF)-kappaB activation, but not by its cytotoxic action. Piceatannol inhibited IkappaB kinase (IKK)-alpha and beta phosphorylation, and subsequently IkappaB-alpha phosphorylation in LPS-stimulated RAW 264.7 cells. On the other hand, piceatannol did not affect activation of mitogen-activated protein (MAP) kinases including extracellular signal regulated kinase 1/2 (Erk1/2), p38 and stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK). Piceatannol inhibited the phosphorylation of Akt and Raf-1 molecules, which regulated the activation of IKK-alpha and beta phosphorylation. The detailed mechanism of the inhibition of LPS-induced NO production by piceatannol is discussed.
Volume 48(10)
Pages 729-36
Published 2004-1-1
DOI 10.1111/j.1348-0421.2004.tb03598.x
PII JST.JSTAGE/mandi/48.729
PMID 15502405
MeSH Animals Cell Line I-kappa B Kinase Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / metabolism Lipopolysaccharides / pharmacology* Macrophages / drug effects* Macrophages / immunology Macrophages / metabolism Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism* Nitric Oxide / antagonists & inhibitors Nitric Oxide / biosynthesis* Protein Serine-Threonine Kinases / antagonists & inhibitors* Signal Transduction Stilbenes / pharmacology* p38 Mitogen-Activated Protein Kinases / metabolism
IF 1.566
Human and Animal Cells RAW 264(RCB0535)