| RRC ID |
73191
|
| 著者 |
Islam S, Hassan F, Mu MM, Ito H, Koide N, Mori I, Yoshida T, Yokochi T.
|
| タイトル |
Piceatannol prevents lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor (NF)-kappaB activation by inhibiting IkappaB kinase (IKK).
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
The effect of piceatannol on lipopolysaccharide (LPS)-induced nitric oxide (NO) production was examined. Piceatannol significantly inhibited NO production in LPS-stimulated RAW 264.7 cells. The inhibition was due to the reduced expression of an inducible isoform of NO synthase (iNOS). The inhibitory effect of piceatannol was mediated by down-regulation of LPS-induced nuclear factor (NF)-kappaB activation, but not by its cytotoxic action. Piceatannol inhibited IkappaB kinase (IKK)-alpha and beta phosphorylation, and subsequently IkappaB-alpha phosphorylation in LPS-stimulated RAW 264.7 cells. On the other hand, piceatannol did not affect activation of mitogen-activated protein (MAP) kinases including extracellular signal regulated kinase 1/2 (Erk1/2), p38 and stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK). Piceatannol inhibited the phosphorylation of Akt and Raf-1 molecules, which regulated the activation of IKK-alpha and beta phosphorylation. The detailed mechanism of the inhibition of LPS-induced NO production by piceatannol is discussed.
|
| 巻・号 |
48(10)
|
| ページ |
729-36
|
| 公開日 |
2004-1-1
|
| DOI |
10.1111/j.1348-0421.2004.tb03598.x
|
| PII |
JST.JSTAGE/mandi/48.729
|
| PMID |
15502405
|
| MeSH |
Animals
Cell Line
I-kappa B Kinase
Lipopolysaccharides / antagonists & inhibitors
Lipopolysaccharides / metabolism
Lipopolysaccharides / pharmacology*
Macrophages / drug effects*
Macrophages / immunology
Macrophages / metabolism
Mitogen-Activated Protein Kinases / metabolism
NF-kappa B / metabolism*
Nitric Oxide / antagonists & inhibitors
Nitric Oxide / biosynthesis*
Protein Serine-Threonine Kinases / antagonists & inhibitors*
Signal Transduction
Stilbenes / pharmacology*
p38 Mitogen-Activated Protein Kinases / metabolism
|
| IF |
1.566
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
