| RRC ID |
73286
|
| Author |
Yokoo H, Misawa T, Kato T, Tanaka M, Demizu Y, Oba M.
|
| Title |
Development of delivery carriers for plasmid DNA by conjugation of a helical template to oligoarginine.
|
| Journal |
Bioorg Med Chem
|
| Abstract |
Arginine (Arg)-rich peptides can penetrate the cell membrane and deliver nucleic acid-based therapeutics into cells. In this study, a helical template designed with a repeating sequence composed of two l-leucines (l-Leu) and a 2-aminoisobutyric acid (Aib) (l-Leu-l-Leu-Aib) was conjugated to nona-arginine on either the C- or N- terminus, designated as Block 1 and Block 2. Each terminal modification induced helical structure formation and improved the physicochemical properties of peptide/plasmid DNA (pDNA) complexes, resulting in efficient intracellular pDNA delivery. The introduction of a helical template may be effective for the endosomal escape of pDNA and pDNA release from complexes in cells. These results emphasized the potency of a helical template for the development of novel cell-penetrating peptides for pDNA delivery.
|
| Volume |
72
|
| Pages |
116997
|
| Published |
2022-10-15
|
| DOI |
10.1016/j.bmc.2022.116997
|
| PII |
S0968-0896(22)00390-X
|
| PMID |
36088811
|
| MeSH |
Arginine / chemistry
Cell-Penetrating Peptides* / chemistry
DNA / genetics
Leucine
Nucleic Acids*
Peptides
Plasmids
|
| IF |
3.073
|
| Resource |
| DNA material |
pCAcc-Luc+ (RDB02443) |
