Reference - Detail
| RRC ID | 73341 |
|---|---|
| Author | Topal S, Vasseur P, Radman-Livaja M, Peterson CL. |
| Title | Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast. |
| Journal | Nat Commun |
| Abstract |
Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate the impact of H3K56Ac on transcription throughout the yeast cell cycle. We find that H3K56Ac is a genome-wide activator of transcription. While H3K56Ac has a major impact on transcription initiation, it also appears to promote elongation and/or termination. In contrast, H3K56Ac represses promiscuous transcription that occurs immediately following replication fork passage, in this case by promoting efficient nucleosome assembly. We also detect a stepwise increase in transcription as cells transit S phase and enter G2, but this response to increased gene dosage does not require H3K56Ac. Thus, a single histone mark can exert both positive and negative impacts on transcription that are coupled to different cell cycle events. |
| Volume | 10(1) |
| Pages | 4372 |
| Published | 2019-9-26 |
| DOI | 10.1038/s41467-019-12400-5 |
| PII | 10.1038/s41467-019-12400-5 |
| PMID | 31558720 |
| PMC | PMC6763489 |
| MeSH | Acetylation Cell Cycle / genetics* Chromatin Assembly and Disassembly / genetics Histone Code / genetics Histones / genetics* Histones / metabolism Lysine / metabolism Nucleosomes / genetics Nucleosomes / metabolism Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism Transcriptional Activation* |
| IF | 12.121 |
| Resource | |
| Yeast | FY17156 |