論文 - 詳細
| RRC ID | 73410 |
|---|---|
| 著者 | Takahashi H, Hotta K, Takagi C, Ueno N, Satoh N, Shoguchi E. |
| タイトル | Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. |
| ジャーナル | Zoolog Sci |
| Abstract |
Brachyury, a T-box transcription factor, is expressed in ascidian embryos exclusively in primordial notochord cells and plays a pivotal role in differentiation of notochord cells. Previously, we identified approximately 450 genes downstream of Ciona intestinalis Brachyury (Ci-Bra), and characterized the expression profiles of 45 of these in differentiating notochord cells. In this study, we looked for cisregulatory sequences in minimal enhancers of 20 Ci-Bra downstream genes by electroporating region within approximately 3 kb upstream of each gene fused with lacZ. Eight of the 20 reporters were expressed in notochord cells. The minimal enchancer for each of these eight genes was narrowed to a region approximately 0.5-1.0-kb long. We also explored the genome-wide and coordinate regulation of 43 Ci-Bra-downstream genes. When we determined their chromosomal localization, it became evident that they are not clustered in a given region of the genome, but rather distributed evenly over 13 of the 14 pairs of chromosomes, suggesting that gene clustering does not contribute to coordinate control of the Ci-Bra downstream gene expression. Our results might provide Insights Into the molecular mechanisms underlying notochord formation in chordates. |
| 巻・号 | 27(2) |
| ページ | 110-8 |
| 公開日 | 2010-2-1 |
| DOI | 10.2108/zsj.27.110 |
| PMID | 20141416 |
| MeSH | Animals Ciona intestinalis / embryology* Gene Expression Profiling Gene Expression Regulation, Developmental / physiology* Genome Notochord / metabolism* |
| IF | 0.843 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |