RRC ID 73416
Author Satouh Y, Inaba K.
Title Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain.
Journal FEBS Lett
Abstract Radial spokes are T-shaped protein complexes important for the regulation of axonemal dyneins in eukaryotic cilia and flagella. Using a functional proteomics approach, we identified six spoke proteins in sperm flagella of the ascidian Ciona intestinalis. Many of the domain/motif structures in spoke proteins are commonly found in flagella of both Ciona sperm and Chlamydomonas, but interestingly they often distribute over non-orthologous protein components. A novel 116 kDa protein named CMUB116 has both an ubiquitin domain and an IQ motif. It has orthologs in vertebrates, but not in Chlamydomonas. Furthermore, the results obtained by immunological analysis provide strong indication that CMUB116 is located at the stalk of radial spokes, where it is associated with MORN40.
Volume 583(13)
Pages 2201-7
Published 2009-7-7
DOI 10.1016/j.febslet.2009.06.016
PII S0014-5793(09)00462-1
PMID 19527718
MeSH Animals Axoneme / chemistry Carrier Proteins / chemistry* Ciona intestinalis / metabolism DNA, Complementary Immunohistochemistry Male Models, Genetic Peptide Mapping Proteomics Protozoan Proteins / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sperm Tail / chemistry* Ubiquitin / chemistry* Ubiquitin / metabolism
IF 3.057
C.intestinalis / (O.japonicus) Wild C. int