論文 - 詳細
| RRC ID | 73451 |
|---|---|
| 著者 | Oshikawa M, Sugai Y, Usami R, Ohtoko K, Toyama S, Kato S. |
| タイトル | Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the vector-capping method. |
| ジャーナル | DNA Res |
| Abstract |
Recently, we have developed a vector-capping method for constructing a full-length cDNA library. In the present study, we performed in-depth analysis of the vector-capped cDNA library prepared from a single type of cell. As a result of single-pass sequencing analysis of 24,000 clones randomly isolated from the unamplified library, we identified 19,951 full-length cDNA clones whose intactness was confirmed by the presence of an additional G at their 5' end. The full-length cDNA content was >95%. Mapping these sequences to the human genome, we identified 4,513 transcriptional units that include 36 antisense transcripts against known genes. Comparison of the frequencies of abundant clones showed that the expression profiles of different libraries, including the distribution of transcriptional start sites (TSSs), were reproducible. The analysis of long-sized cDNAs showed that this library contained many cDNAs with a long-sized insert up to 11,199 bp of golgin B, including multiple slicing variants for filamin A and filamin B. These results suggest that the size-unbiased full-length cDNA library constructed using the vector-capping method will be an ideal resource for fine expression profiling of transcriptional variants with alternative TSSs and alternative splicing. |
| 巻・号 | 15(3) |
| ページ | 123-36 |
| 公開日 | 2008-6-30 |
| DOI | 10.1093/dnares/dsn010 |
| PII | dsn010 |
| PMID | 18487259 |
| PMC | PMC2650634 |
| MeSH | Cells, Cultured Chromosome Mapping Cloning, Molecular Databases, Nucleic Acid Gene Dosage Gene Expression Profiling / methods* Gene Library* Genetic Vectors* / physiology Humans RNA Caps / chemistry RNA Caps / genetics* RNA, Messenger / analysis* RNA, Messenger / chemistry RNA, Messenger / isolation & purification Reproducibility of Results Transcription Initiation Site / physiology |
| IF | 4.009 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | NRCD human cDNA clones (RDB06607) |