RRC ID 73492
Author Sawamura K, Maehara K, Mashino S, Kagesawa T, Kajiwara M, Matsuno K, Takahashi A, Takano-Shimizu T.
Title Introgression of Drosophila simulans nuclear pore protein 160 in Drosophila melanogaster alone does not cause inviability but does cause female sterility.
Journal Genetics
Abstract We have been analyzing genes for reproductive isolation by replacing Drosophila melanogaster genes with homologs from Drosophila simulans by interspecific backcrossing. Among the introgressions established, we found that a segment of the left arm of chromosome 2, Int(2L)S, carried recessive genes for hybrid sterility and inviability. That nuclear pore protein 160 (Nup160) in the introgression region is involved in hybrid inviability, as suggested by others, was confirmed by the present analysis. Male hybrids carrying an X chromosome of D. melanogaster were not rescued by the Lethal hybrid rescue (Lhr) mutation when the D. simulans Nup160 allele was made homozygous or hemizygous. Furthermore, we uniquely found that Nup160 is also responsible for hybrid sterility. Females were sterile when D. simulans Nup160 was made homozygous or hemizygous in the D. melanogaster genetic background. Genetic analyses indicated that the D. simulans Nup160 introgression into D. melanogaster was sufficient to cause female sterility but that other autosomal genes of D. simulans were also necessary to cause lethality. The involvement of Nup160 in hybrid inviability and female sterility was confirmed by transgene experiment.
Volume 186(2)
Pages 669-76
Published 2010-10-1
DOI 10.1534/genetics.110.119867
PII genetics.110.119867
PMID 20647504
PMC PMC2954468
MeSH Animals Animals, Genetically Modified Chromosomes, Insect Crosses, Genetic Drosophila / genetics* Drosophila / physiology Drosophila Proteins / genetics Drosophila melanogaster / genetics* Drosophila melanogaster / physiology Female Genes, Insect* Genes, Lethal Genetic Engineering Genetic Markers Genetic Variation Genome Genotype Heterozygote Hybridization, Genetic Infertility, Female / genetics Male Nuclear Pore Complex Proteins / genetics* Nuclear Proteins / genetics Phenotype Polymerase Chain Reaction Transgenes
IF 4.015
Drosophila DGRC#118826