論文 - 詳細
| RRC ID | 73667 |
|---|---|
| 著者 | Tanaka S. |
| タイトル | An efficient method for the isolation of interaction-null/impaired mutants using the yeast two-hybrid technique. |
| ジャーナル | Genes Cells |
| Abstract |
Protein-protein interactions are one of the most basic and critical processes underlying biological functions. Thus, identification of the interacting proteins of a protein of interest and further elucidation of the roles of the interactions is critical for understanding the related biological processes. The yeast two-hybrid (Y2H) method is a popular approach for identifying protein-protein interactions. Once interacting proteins are identified, a comparison of the phenotypes of mutants lacking the specific protein-protein interaction with those of the wild-type strain is a powerful tool for uncovering the former interactions' biological significance. However, isolation of such interaction-defective mutants is often laborious. Here, I describe a novel and efficient approach for isolating such mutants that uses the Y2H technique with a modified Y2H vector, and provide an example of how this approach can be used to screen interaction-null/impaired mutants. Because the strategy is simple and the modification of a pre-existing Y2H vector is sufficient for the screening purpose, the same strategy can be applied to any existing two-hybrid system. |
| 巻・号 | 24(12) |
| ページ | 781-788 |
| 公開日 | 2019-12-1 |
| DOI | 10.1111/gtc.12724 |
| PMID | 31599053 |
| MeSH | Binding Sites / genetics Mutation* Protein Binding Saccharomyces cerevisiae Two-Hybrid System Techniques* |
| IF | 1.655 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BYP9683 (pST2303) BYP9682 (pST2302) |