論文 - 詳細
| RRC ID | 73836 |
|---|---|
| 著者 | Matos-Perdomo E, Santana-Sosa S, Ayra-Plasencia J, Medina-Suárez S, Machín F. |
| タイトル | The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delays. |
| ジャーナル | Life Sci Alliance |
| Abstract |
The ribosomal DNA (rDNA) array of Saccharomyces cerevisiae has served as a model to address chromosome organization. In cells arrested before anaphase (mid-M), the rDNA acquires a highly structured chromosomal organization referred to as the rDNA loop, whose length can double the cell diameter. Previous works established that complexes such as condensin and cohesin are essential to attain this structure. Here, we report that the rDNA loop adopts distinct presentations that arise as spatial adaptations to changes in the nuclear morphology triggered during mid-M arrests. Interestingly, the formation of the rDNA loop results in the appearance of a space under the loop (SUL) which is devoid of nuclear components yet colocalizes with the vacuole. We show that the rDNA-associated nuclear envelope (NE) often reshapes into a ladle to accommodate the vacuole in the SUL, with the nucleus becoming bilobed and doughnut-shaped. Finally, we demonstrate that the formation of the rDNA loop and the SUL require TORC1, membrane synthesis and functional vacuoles, yet is independent of nucleus-vacuole junctions and rDNA-NE tethering. |
| 巻・号 | 5(10) |
| 公開日 | 2022-10-1 |
| DOI | 10.26508/lsa.202101161 |
| PII | 5/10/e202101161 |
| PMID | 35961781 |
| PMC | PMC9375157 |
| MeSH | Anaphase DNA, Ribosomal / genetics DNA, Ribosomal / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Vacuoles* / metabolism |
| IF | 2.622 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BYP6849(pMK42) BYP6850(pMK72) BY25598(YNK54) |