論文 - 詳細
RRC ID | 73947 |
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著者 | Inouye S. |
タイトル | NAD(P)H-flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, is a flavoprotein. |
ジャーナル | FEBS Lett |
Abstract |
The NAD(P)H-flavin oxidoreductase gene from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, was expressed in Escherichia coli, and the enzyme purified using Cibacron Blue 3G-A affinity column chromatography from crude extracts in a single step. The purified enzyme had a typical flavoprotein absorption spectrum and flavin mononucleotide (FMN) was identified as a prosthetic group, non-covalently bound in a molar ratio of 1:1. The enzyme catalyzed the electron transfer from NADH via FMNH2 to various other electron acceptors. Reduced flavin produced by flavin reductase participated non-enzymatically in the following reactions: H2O2-forming NADH oxidase-like, oxygen-insensitive nitroreductase-like, diaphorase (quinone reductase)-like and bacterial luciferase reactions. |
巻・号 | 347(2-3) |
ページ | 163-8 |
公開日 | 1994-6-27 |
DOI | 10.1016/0014-5793(94)00528-1 |
PII | 0014-5793(94)00528-1 |
PMID | 8033996 |
MeSH | Amino Acid Sequence Chromatography, Affinity Electron Transport Electrophoresis, Polyacrylamide Gel Enzyme Stability Escherichia coli FMN Reductase Flavin Mononucleotide / analysis Flavin Mononucleotide / chemistry Flavin Mononucleotide / metabolism Hot Temperature Hydrogen Peroxide / metabolism Hydrogen-Ion Concentration Luminescence Molecular Sequence Data Molecular Weight NAD / metabolism NADH, NADPH Oxidoreductases / chemistry NADH, NADPH Oxidoreductases / genetics NADH, NADPH Oxidoreductases / metabolism* Recombinant Proteins Spectrophotometry Vibrio / enzymology* |
IF | 3.057 |
リソース情報 | |
遺伝子材料 | pJN-FRase (RDB19835) |