| RRC ID |
74
|
| 著者 |
Wang A, Nishihashi T, Murakami S, Trandafir CC, Ji X, Shimizu Y, Kurahashi K.
|
| タイトル |
Noradrenaline-induced contraction mediated by endothelial COX-1 metabolites in the rat coronary artery.
|
| ジャーナル |
J Cardiovasc Pharmacol
|
| Abstract |
Noradrenaline-induced contraction of the rat coronary arteries was significantly augmented by the presence of NG-nitro-L-arginine methyl ester (L-NAME) and arachidonic acid. The experiments in the study presented here were undertaken to characterize pharmacologically the augmented noradrenaline-induced contraction in ring preparations of rat coronary arteries. The contraction was stopped by a chemical remover of endothelium (saponin). Oxygen radical scavengers, superoxide dismutase and catalase, significantly attenuated the contraction. Cyclooxygenase-1 inhibitors (flurbiprofen, 10(-7) M) attenuated the noradrenaline-induced contraction and cyclooxygenase-2 (nimesulide, 10(-7) M) slightly attenuated the contraction. A thromboxane A2 (TXA2) synthetase inhibitor (OKY-046) and a TXA2 receptor antagonist (S-1452) did not affect the contraction. Based on these results, it was suggested that the contraction induced by noradrenaline in the rat coronary artery in the presence of L-NAME and arachidonic acid is endothelium-dependent, and that it involves reactive oxygen species and endothelial cyclooxygenase-1 metabolites of arachidonic acid.
|
| 巻・号 |
42 Suppl 1
|
| ページ |
S39-42
|
| 公開日 |
2003-12-1
|
| DOI |
10.1097/00005344-200312001-00010
|
| PMID |
14871027
|
| MeSH |
Animals
Arachidonic Acid / administration & dosage
Arachidonic Acid / metabolism
Arachidonic Acid / pharmacokinetics
Coronary Vessels / drug effects*
Cyclooxygenase 1
Dose-Response Relationship, Drug
Drug Synergism
Endothelium, Vascular / drug effects
Endothelium, Vascular / enzymology*
Endothelium, Vascular / physiology
Flurbiprofen / administration & dosage
Flurbiprofen / pharmacokinetics
Free Radical Scavengers / administration & dosage
Free Radical Scavengers / pharmacokinetics
Isoenzymes / metabolism*
Male
Membrane Proteins
Muscle Contraction / drug effects*
Muscle, Smooth, Vascular / drug effects
NG-Nitroarginine Methyl Ester / administration & dosage
NG-Nitroarginine Methyl Ester / pharmacokinetics
Norepinephrine / administration & dosage
Norepinephrine / antagonists & inhibitors
Norepinephrine / pharmacokinetics*
Prostaglandin-Endoperoxide Synthases / metabolism*
Rats
Rats, Wistar
Reactive Oxygen Species / metabolism
Saponins / administration & dosage
Saponins / pharmacokinetics
|
| IF |
2.598
|
| 引用数 |
4
|
|
WOS 分野
|
CARDIAC & CARDIOVASCULAR SYSTEMS
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ラット |
|
