Author Wang A, Nishihashi T, Murakami S, Trandafir CC, Ji X, Shimizu Y, Kurahashi K.
Title Noradrenaline-induced contraction mediated by endothelial COX-1 metabolites in the rat coronary artery.
Journal J. Cardiovasc. Pharmacol.
Abstract Noradrenaline-induced contraction of the rat coronary arteries was significantly augmented by the presence of NG-nitro-L-arginine methyl ester (L-NAME) and arachidonic acid. The experiments in the study presented here were undertaken to characterize pharmacologically the augmented noradrenaline-induced contraction in ring preparations of rat coronary arteries. The contraction was stopped by a chemical remover of endothelium (saponin). Oxygen radical scavengers, superoxide dismutase and catalase, significantly attenuated the contraction. Cyclooxygenase-1 inhibitors (flurbiprofen, 10(-7) M) attenuated the noradrenaline-induced contraction and cyclooxygenase-2 (nimesulide, 10(-7) M) slightly attenuated the contraction. A thromboxane A2 (TXA2) synthetase inhibitor (OKY-046) and a TXA2 receptor antagonist (S-1452) did not affect the contraction. Based on these results, it was suggested that the contraction induced by noradrenaline in the rat coronary artery in the presence of L-NAME and arachidonic acid is endothelium-dependent, and that it involves reactive oxygen species and endothelial cyclooxygenase-1 metabolites of arachidonic acid.
Volume 42 Suppl 1
Pages S39-42
Published 2003-12
PMID 14871027
MeSH Animals Arachidonic Acid / administration & dosage Arachidonic Acid / metabolism Arachidonic Acid / pharmacokinetics Coronary Vessels / drug effects* Cyclooxygenase 1 Dose-Response Relationship, Drug Drug Synergism Endothelium, Vascular / drug effects Endothelium, Vascular / enzymology* Endothelium, Vascular / physiology Flurbiprofen / administration & dosage Flurbiprofen / pharmacokinetics Free Radical Scavengers / administration & dosage Free Radical Scavengers / pharmacokinetics Isoenzymes / metabolism* Male Membrane Proteins Muscle Contraction / drug effects* Muscle, Smooth, Vascular / drug effects NG-Nitroarginine Methyl Ester / administration & dosage NG-Nitroarginine Methyl Ester / pharmacokinetics Norepinephrine / administration & dosage Norepinephrine / antagonists & inhibitors Norepinephrine / pharmacokinetics* Prostaglandin-Endoperoxide Synthases / metabolism* Rats Rats, Wistar Reactive Oxygen Species / metabolism Saponins / administration & dosage Saponins / pharmacokinetics
IF 2.227
Times Cited 3