RRC ID |
74134
|
Author |
Tomihara K, Katsuma S, Kiuchi T.
|
Title |
Transcriptome analysis in the silkworm Bombyx mori overexpressing piRNA-resistant Masculinizer gene.
|
Journal |
Biochem Biophys Res Commun
|
Abstract |
Dosage compensation is a process that produces a similar expression of sex-linked and autosomal genes. In the silkworm Bombyx mori with a WZ sex-determination system, the expression from the single Z in WZ females matches that of ZZ males due to the suppression of Z-linked genes in males. A primary maleness determinant gene, Masculinizer (Masc), is also required for dosage compensation. In females, silkworm Piwi is complexed with the W chromosome-derived female-specific Feminizer (Fem) PIWI-interacting RNA (piRNA) and cleaves Masc mRNA. When Fem piRNA-resistant Masc cDNA (Masc-R) is overexpressed in both sexes, only female larvae are dead during the larval stage. In this study, transcriptome analysis was performed in neonate larvae to examine the effects of Masc-R overexpression on a global gene expression profile. Z-linked genes were globally repressed in Masc-R-overexpressing females due to force-driven dosage compensation. In contrast, Masc-R overexpression had little effect on the expression of Z-linked genes and the male-specific isoform of B. mori insulin-like growth factor II mRNA-binding protein in males, indicating that excessive Masc expression strengthens neither dosage compensation nor maleness in males. Fourteen genes were differentially expressed between Masc-R-overexpressing and control neonate larvae in both sexes, suggesting Masc functions other than dosage compensation and masculinization.
|
Volume |
616
|
Pages |
104-109
|
Published |
2022-8-6
|
DOI |
10.1016/j.bbrc.2022.05.073
|
PII |
S0006-291X(22)00784-7
|
PMID |
35653824
|
MeSH |
Animals
Bombyx* / metabolism
Female
Gene Expression Profiling
Humans
Infant, Newborn
Insect Proteins / genetics
Insect Proteins / metabolism
Male
RNA, Messenger / genetics
RNA, Small Interfering / genetics
|
IF |
2.985
|
Resource |
Silkworms |
p50 |