論文 - 詳細
| RRC ID | 74134 |
|---|---|
| 著者 | Tomihara K, Katsuma S, Kiuchi T. |
| タイトル | Transcriptome analysis in the silkworm Bombyx mori overexpressing piRNA-resistant Masculinizer gene. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Dosage compensation is a process that produces a similar expression of sex-linked and autosomal genes. In the silkworm Bombyx mori with a WZ sex-determination system, the expression from the single Z in WZ females matches that of ZZ males due to the suppression of Z-linked genes in males. A primary maleness determinant gene, Masculinizer (Masc), is also required for dosage compensation. In females, silkworm Piwi is complexed with the W chromosome-derived female-specific Feminizer (Fem) PIWI-interacting RNA (piRNA) and cleaves Masc mRNA. When Fem piRNA-resistant Masc cDNA (Masc-R) is overexpressed in both sexes, only female larvae are dead during the larval stage. In this study, transcriptome analysis was performed in neonate larvae to examine the effects of Masc-R overexpression on a global gene expression profile. Z-linked genes were globally repressed in Masc-R-overexpressing females due to force-driven dosage compensation. In contrast, Masc-R overexpression had little effect on the expression of Z-linked genes and the male-specific isoform of B. mori insulin-like growth factor II mRNA-binding protein in males, indicating that excessive Masc expression strengthens neither dosage compensation nor maleness in males. Fourteen genes were differentially expressed between Masc-R-overexpressing and control neonate larvae in both sexes, suggesting Masc functions other than dosage compensation and masculinization. |
| 巻・号 | 616 |
| ページ | 104-109 |
| 公開日 | 2022-8-6 |
| DOI | 10.1016/j.bbrc.2022.05.073 |
| PII | S0006-291X(22)00784-7 |
| PMID | 35653824 |
| MeSH | Animals Bombyx* / metabolism Female Gene Expression Profiling Humans Infant, Newborn Insect Proteins / genetics Insect Proteins / metabolism Male RNA, Messenger / genetics RNA, Small Interfering / genetics |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50 |