RRC ID 74134
Author Tomihara K, Katsuma S, Kiuchi T.
Title Transcriptome analysis in the silkworm Bombyx mori overexpressing piRNA-resistant Masculinizer gene.
Journal Biochem Biophys Res Commun
Abstract Dosage compensation is a process that produces a similar expression of sex-linked and autosomal genes. In the silkworm Bombyx mori with a WZ sex-determination system, the expression from the single Z in WZ females matches that of ZZ males due to the suppression of Z-linked genes in males. A primary maleness determinant gene, Masculinizer (Masc), is also required for dosage compensation. In females, silkworm Piwi is complexed with the W chromosome-derived female-specific Feminizer (Fem) PIWI-interacting RNA (piRNA) and cleaves Masc mRNA. When Fem piRNA-resistant Masc cDNA (Masc-R) is overexpressed in both sexes, only female larvae are dead during the larval stage. In this study, transcriptome analysis was performed in neonate larvae to examine the effects of Masc-R overexpression on a global gene expression profile. Z-linked genes were globally repressed in Masc-R-overexpressing females due to force-driven dosage compensation. In contrast, Masc-R overexpression had little effect on the expression of Z-linked genes and the male-specific isoform of B. mori insulin-like growth factor II mRNA-binding protein in males, indicating that excessive Masc expression strengthens neither dosage compensation nor maleness in males. Fourteen genes were differentially expressed between Masc-R-overexpressing and control neonate larvae in both sexes, suggesting Masc functions other than dosage compensation and masculinization.
Volume 616
Pages 104-109
Published 2022-8-6
DOI 10.1016/j.bbrc.2022.05.073
PII S0006-291X(22)00784-7
PMID 35653824
MeSH Animals Bombyx* / metabolism Female Gene Expression Profiling Humans Infant, Newborn Insect Proteins / genetics Insect Proteins / metabolism Male RNA, Messenger / genetics RNA, Small Interfering / genetics
IF 2.985
Silkworms p50