| RRC ID |
74145
|
| 著者 |
Nerome K, Imagawa T, Sugita S, Arasaki Y, Maegawa K, Kawasaki K, Tanaka T, Watanabe S, Nishimura H, Suzuki T, Kuroda K, Kosugi I, Kajiura Z.
|
| タイトル |
The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokine.
|
| ジャーナル |
Life Sci Alliance
|
| Abstract |
The efficacy of the current influenza vaccines is frequently reduced because of antigenic drift, a trade-off of developing improved vaccines with broad cross-protective activity against influenza A viruses. In this study, we have successfully constructed a chimeric cytokine (CC) comprising the M2 protein, influenza A neuraminidase stalk, and interleukin-12. We produced virus-like particles (VLPs) containing CC and influenza hemagglutinin (HA) proteins using a baculovirus system in Eri silkworm pupae. The protective efficacy of the CCHA-VLP vaccine was evaluated in mice. The CCFkH5HA-VLP vaccine increased the survival rates of BALB/c mice, infected with a lethal dose of PRH1 and HKH5 viruses, to 80% and 100%, respectively. The results suggested that CCHA-VLP successfully induced potent cross-reactive protective immunity against infection with homologous and heterologous subtypes of the influenza A virus. This is the first study to design a CC-containing HA-VLP vaccine and validate its protective efficacy.
|
| 巻・号 |
6(1)
|
| 公開日 |
2022-11-7
|
| DOI |
10.26508/lsa.202201548
|
| PII |
6/1/e202201548
|
| PMID |
36344085
|
| PMC |
PMC9644419
|
| MeSH |
Animals
Antibodies, Viral
Cytokines
Hemagglutinins
Humans
Influenza Vaccines*
Influenza, Human* / prevention & control
Mice
Mice, Inbred BALB C
Orthomyxoviridae Infections* / prevention & control
Vaccines, Virus-Like Particle* / genetics
|
| IF |
2.622
|
| リソース情報 |
| カイコ |
Eri silkworm |
