RRC ID 74264
Author Oiki S, Yaguchi T, Urayama SI, Hagiwara D.
Title Wide distribution of resistance to the fungicides fludioxonil and iprodione in Penicillium species.
Journal PLoS One
Abstract Fludioxonil and iprodione are effective fungicides widely used for crop protection and are essential for controlling plant pathogenic fungi. The emergence of fungicide-resistant strains of targeted pathogens is regularly monitored, and several cases have been reported. Non-targeted fungi may also be exposed to the fungicide residues in agricultural fields. However, there are no comprehensive reports on fungicide-resistant strains of non-targeted fungi. Here, we surveyed 99 strains, representing 12 Penicillium species, that were isolated from a variety of environments, including foods, dead bodies, and clinical samples. Among the Penicillium strains, including non-pathogenic P. chrysogenum and P. camembertii, as well as postharvest pathogens P. expansum and P. digitatum, 14 and 20 showed resistance to fludioxonil and iprodione, respectively, and 6 showed multi-drug resistance to the fungicides. Sequence analyses revealed that some strains of P. chrysogenum and Penicillium oxalicum had mutations in NikA, a group III histidine kinase of the high-osmolarity glycerol pathway, which is the mode of action for fludioxonil and iprodione. The single nucleotide polymorphisms of G693D and T1318P in P. chrysogenum and T960S in P. oxalicum were only present in the fludioxonil- or iprodione-resistant strains. These strains also exhibited resistance to pyrrolnitrin, which is the lead compound in fludioxonil and is naturally produced by some Pseudomonas species. This study demonstrated that non-targeted Penicillium strains distributed throughout the environment possess fungicide resistance.
Volume 17(1)
Pages e0262521
Published 2022-1-1
DOI 10.1371/journal.pone.0262521
PII PONE-D-21-28823
PMID 35100282
PMC PMC8803201
MeSH Aminoimidazole Carboxamide / analogs & derivatives* Aminoimidazole Carboxamide / pharmacology Cadaver Crops, Agricultural / microbiology Dioxoles / pharmacology* Drug Resistance, Fungal* Food Analysis Fungal Proteins / genetics* Fungicides, Industrial / pharmacology Humans Hydantoins / pharmacology* Mycoses / drug therapy* Mycoses / genetics Mycoses / microbiology Penicillium / drug effects Penicillium / genetics Penicillium / isolation & purification* Polymorphism, Single Nucleotide* Pyrroles / pharmacology*
IF 2.74
Resource
Pathogenic eukaryotic microorganisms Penicillium brasilanum IFM 42077 IFM 60071 IFM 60072 P. camemberti IFM 54179 IFM 61933 P. chrysogenum IFM 40614 IFM 47768 IFM 52203 IFM 52204 IFM 56829 IFM 57112 IFM 59766 IFM 60605 IFM 60953 P. decumbens IFM 46582 IFM 63512 P. digitatum IFM 60598 IFM 63755 P. expansum IFM 40618 IFM 52210 IFM 62049 P. griseofulvum IFM 54187 IFM 54314 P. italicum IFM 52160 P. oxalicum IFM 54751 IFM 55886 IFM 62137 IFM 62937 P. roqueforti IFM 48063 IFM 58915 NBRC 5956 P. steckii IFM 62327 IFM 63697 IFM 64403 IFM 64664