論文 - 詳細
| RRC ID | 74362 |
|---|---|
| 著者 | Hong J, Sanjoba C, Fujii W, Yamagishi J, Goto Y. |
| タイトル | Leishmania infection-induced multinucleated giant cell formation via upregulation of ATP6V0D2 expression. |
| ジャーナル | Front Cell Infect Microbiol |
| Abstract |
Leishmaniasis is caused by infection with protozoan parasites of the genus Leishmania. In both clinical and experimental visceral leishmaniasis, macrophage multinucleation is observed in parasitized tissues. However, the feature and the mechanism of macrophage multinucleation remained unclear. Here, we report that infection of Leishmania donovani, a causative agent of visceral leishmaniasis, induces multinucleation of bone marrow-derived macrophages (BMDMs) in vitro. When these infection-induced multinucleated macrophages were compared with cytokine-induced multinucleated giant cells, the former had higher phagocytic activity on red blood cells but no apparent changes on phagocytosis of latex beads. BMDMs infected with L. donovani had increased expression of ATP6V0D2, one of the components of V-ATPase, which was also upregulated in the spleen of infected mice. Infection-induced ATP6V0D2 localized in a cytoplasmic compartment, which did not overlap with the mitochondria, endoplasmic reticulum, or lysosomes. When ATP6V0D2 expression was recombinantly induced in BMDMs, the formation of multinucleated macrophages was induced as seen in the infected macrophages. Taken together, L. donovani infection induces multinucleation of macrophages via ATP6V0D2 upregulation leading to a unique metamorphosis of the macrophages toward hemophagocytes. |
| 巻・号 | 12 |
| ページ | 953785 |
| 公開日 | 2022-1-1 |
| DOI | 10.3389/fcimb.2022.953785 |
| PMID | 36211967 |
| PMC | PMC9539756 |
| MeSH | Adenosine Triphosphatases Animals Cytokines Giant Cells Leishmania donovani* Leishmaniasis, Visceral* / parasitology Mice Mice, Inbred BALB C Up-Regulation Vacuolar Proton-Translocating ATPases* |
| IF | 4.123 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原真核微生物 | Leishmania donovani Ld003 |