RRC ID 74422
Author Hashino T, Matsubara H, Xu J, Tanaka R, Kusagawa E, Ueda Y, Yoshida H, Kataoka T.
Title PGAM5 interacts with Bcl-rambo and regulates apoptosis and mitophagy.
Journal Exp Cell Res
Abstract Bcl-rambo, also known as BCL2L13, has been reported to regulate apoptosis, mitochondrial fragmentation, and mitophagy. However, the molecular mechanisms by which Bcl-rambo regulates these processes currently remain unclear. In the present study, we identified phosphoglycerate mutase member 5 (PGAM5) as an emerging partner interacting with Bcl-rambo through phenotypic Drosophila screening. The rough eye phenotype induced by human Bcl-rambo was partly rescued by the knockdown of pgam5-2, a mammalian ortholog of PGAM5. Bcl-rambo bound to PGAM5, and their interaction required the Bcl-rambo transmembrane domain. The co-expression of Bcl-rambo and PGAM5 promoted effector caspase activity in human embryonic kidney 293T cells. The transient overexpression of Bcl-rambo increased LC3B-II levels, which had been decreased by the co-expression of PGAM5. These results suggest that PGAM5 promotes Bcl-rambo-dependent apoptosis, but conversely interferes with Bcl-rambo-dependent mitophagy.
Volume 420(1)
Pages 113342
Published 2022-11-1
DOI 10.1016/j.yexcr.2022.113342
PII S0014-4827(22)00335-4
PMID 36075447
MeSH Animals Apoptosis / genetics Caspases, Effector / metabolism Drosophila / metabolism Humans Mammals / metabolism Mitochondria / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Mitophagy* / genetics Phosphoglycerate Mutase* / metabolism Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism
IF 3.383
Human and Animal Cells K562(RCB0027)