論文 - 詳細
| RRC ID | 74422 |
|---|---|
| 著者 | Hashino T, Matsubara H, Xu J, Tanaka R, Kusagawa E, Ueda Y, Yoshida H, Kataoka T. |
| タイトル | PGAM5 interacts with Bcl-rambo and regulates apoptosis and mitophagy. |
| ジャーナル | Exp Cell Res |
| Abstract |
Bcl-rambo, also known as BCL2L13, has been reported to regulate apoptosis, mitochondrial fragmentation, and mitophagy. However, the molecular mechanisms by which Bcl-rambo regulates these processes currently remain unclear. In the present study, we identified phosphoglycerate mutase member 5 (PGAM5) as an emerging partner interacting with Bcl-rambo through phenotypic Drosophila screening. The rough eye phenotype induced by human Bcl-rambo was partly rescued by the knockdown of pgam5-2, a mammalian ortholog of PGAM5. Bcl-rambo bound to PGAM5, and their interaction required the Bcl-rambo transmembrane domain. The co-expression of Bcl-rambo and PGAM5 promoted effector caspase activity in human embryonic kidney 293T cells. The transient overexpression of Bcl-rambo increased LC3B-II levels, which had been decreased by the co-expression of PGAM5. These results suggest that PGAM5 promotes Bcl-rambo-dependent apoptosis, but conversely interferes with Bcl-rambo-dependent mitophagy. |
| 巻・号 | 420(1) |
| ページ | 113342 |
| 公開日 | 2022-11-1 |
| DOI | 10.1016/j.yexcr.2022.113342 |
| PII | S0014-4827(22)00335-4 |
| PMID | 36075447 |
| MeSH | Animals Apoptosis / genetics Caspases, Effector / metabolism Drosophila / metabolism Humans Mammals / metabolism Mitochondria / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Mitophagy* / genetics Phosphoglycerate Mutase* / metabolism Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism |
| IF | 3.383 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |
| ショウジョウバエ | |