論文 - 詳細
| RRC ID | 74442 |
|---|---|
| 著者 | Kobayashi Y, Watanabe N, Hiura R, Kubota M, Furuta K, Sugimoto K, Murota K, Nakamura E, Matsuura T, Kai K, Inui T, Kitakaze T, Harada N, Yamaji R. |
| タイトル | Transport Form and Pathway from the Intestine to the Peripheral Tissues and the Intestinal Absorption and Metabolism Properties of Oleamide. |
| ジャーナル | J Agric Food Chem |
| Abstract |
This study aimed to obtain information on the transport form and pathway from the intestine to the peripheral tissues and on the intestinal absorption and metabolism properties of oleamide (cis-9-octadecenamide). Oleamide was primarily transported via the portal vein. Density gradient centrifugation indicated that plasma oleamide was enriched in the fractions containing albumin in the portal and peripheral blood. Oleamide formed a complex with albumin in an endothermic reaction (apparent Kd = 4.4 μM). The CD36 inhibitor inhibited the oleamide uptake into the intestinal epithelial Caco-2 cells, and oleamide decreased the cell surface CD36 level. The fatty acid amide hydrolase (FAAH) inhibitor increased the transepithelial transport of oleamide across Caco-2 cells and the plasma oleamide concentration in mice intragastrically administered with oleamide. These results indicate that oleamide is transported primarily via the portal vein as a complex with albumin. Furthermore, we suggest that oleamide is taken up via CD36 in the small intestine and degraded intracellularly by FAAH. |
| 巻・号 | 70(49) |
| ページ | 15499-15508 |
| 公開日 | 2022-12-14 |
| DOI | 10.1021/acs.jafc.2c06791 |
| PMID | 36458736 |
| MeSH | Albumins Animals Caco-2 Cells Humans Intestinal Absorption* Intestine, Small* Mice |
| IF | 4.192 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |