| RRC ID |
74455
|
| 著者 |
Panahipour L, Sordi MB, Kargarpour Z, Gruber R.
|
| タイトル |
TGF-β Signalling Mediates the Anti-Inflammatory Activity of Enamel Matrix Derivative In Vitro.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Enamel matrix derivative (EMD) prepared from extracted porcine fetal tooth material can support the regrow of periodontal tissues. Previous findings suggest that EMD has anti-inflammatory properties and TGF-β activity in vitro. However, the anti-inflammatory activity of EMD is mediated via TGF-β has not been considered. To this aim, we first established a bioassay to confirm the anti-inflammatory activity of EMD. The bioassay was based on the RAW 264.7 macrophage cell line and proven with primary macrophages where EMD significantly reduced the forced expression of IL-6. We then confirmed the presence of TGF-β1 in EMD by immunoassay and by provoking the Smad2/3 nuclear translocation in RAW 264.7 macrophages. Next, we took advantage of the TGF-β receptor type I kinase-inhibitor SB431542 to block the respective signalling pathway. SB431542 reversed the anti-inflammatory activity of EMD and TGF-β in a bioassay when IL-6 and CXCL2 expression was driven by the LPS stimulation of RAW 264.7 macrophages. This central observation was supported by showing that SB431542 reversed the anti-inflammatory activity of EMD using IL-1β and TNF-α-stimulated ST2 bone marrow stromal cells. Together, these findings implicate that the TGF-β activity mediates at least part of the anti-inflammatory activity of EMD in vitro.
|
| 巻・号 |
23(17)
|
| 公開日 |
2022-8-29
|
| DOI |
10.3390/ijms23179778
|
| PII |
ijms23179778
|
| PMID |
36077174
|
| PMC |
PMC9456059
|
| MeSH |
Animals
Anti-Inflammatory Agents / pharmacology
Cells, Cultured
Dental Enamel Proteins* / pharmacology
Interleukin-6*
Swine
Transforming Growth Factor beta
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
