論文 - 詳細
| RRC ID | 74491 |
|---|---|
| 著者 | Uchiyama H, Muramatsu D, Higashi H, Kida H, Iwai A. |
| タイトル | Effects of chondroitin sulfate oligosaccharides on osteoclast differentiation of RAW264 cells, and myotube differentiation of C2C12 cells. |
| ジャーナル | PLoS One |
| Abstract |
Chondroitin sulfate (CS) is a glycosaminoglycan, and CS derived from various animal species is used in drugs and food supplements to alleviate arthralgia. The CS is a high molecular weight compound, and hydrolysis of CS by intestinal microbiota is thought to be required for absorption in mammalians. Chondroitin sulfate oligosaccharides (Oligo-CS) are produced by hydrolysis with subcritical water from CS isolated from a species of skate, Raja pulchra for the improvement of bioavailability. The present study conducted in vitro experiments using murine cell lines, to compare the biological activities of Oligo-CS and high molecular weight CS composed with the similar disaccharide isomer units of D-glucuronic acid and N-acetyl-D-glucosamine (CS-C). The results show that Oligo-CS inhibits osteoclast differentiation of RAW264 cells significantly at lower concentrations than in CS. The cell viability of a myoblast cell line, C2C12 cells, was increased when the cells were grown in a differentiated medium for myotubes with Oligo-CS, where there were no effects on the cell viability in CS. These results suggest that in vitro Oligo-CS exhibits stronger bioactivity than high-molecular weight CS. |
| 巻・号 | 18(4) |
| ページ | e0284343 |
| 公開日 | 2023-1-1 |
| DOI | 10.1371/journal.pone.0284343 |
| PII | PONE-D-22-32503 |
| PMID | 37053208 |
| PMC | PMC10101473 |
| MeSH | Animals Cell Differentiation Chondroitin Sulfates* / metabolism Chondroitin Sulfates* / pharmacology Mammals / metabolism Mice Muscle Fibers, Skeletal / metabolism Oligosaccharides / pharmacology Osteoclasts* / metabolism |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |