RRC ID 74491
著者 Uchiyama H, Muramatsu D, Higashi H, Kida H, Iwai A.
タイトル Effects of chondroitin sulfate oligosaccharides on osteoclast differentiation of RAW264 cells, and myotube differentiation of C2C12 cells.
ジャーナル PLoS One
Abstract Chondroitin sulfate (CS) is a glycosaminoglycan, and CS derived from various animal species is used in drugs and food supplements to alleviate arthralgia. The CS is a high molecular weight compound, and hydrolysis of CS by intestinal microbiota is thought to be required for absorption in mammalians. Chondroitin sulfate oligosaccharides (Oligo-CS) are produced by hydrolysis with subcritical water from CS isolated from a species of skate, Raja pulchra for the improvement of bioavailability. The present study conducted in vitro experiments using murine cell lines, to compare the biological activities of Oligo-CS and high molecular weight CS composed with the similar disaccharide isomer units of D-glucuronic acid and N-acetyl-D-glucosamine (CS-C). The results show that Oligo-CS inhibits osteoclast differentiation of RAW264 cells significantly at lower concentrations than in CS. The cell viability of a myoblast cell line, C2C12 cells, was increased when the cells were grown in a differentiated medium for myotubes with Oligo-CS, where there were no effects on the cell viability in CS. These results suggest that in vitro Oligo-CS exhibits stronger bioactivity than high-molecular weight CS.
巻・号 18(4)
ページ e0284343
公開日 2023-1-1
DOI 10.1371/journal.pone.0284343
PII PONE-D-22-32503
PMID 37053208
PMC PMC10101473
MeSH Animals Cell Differentiation Chondroitin Sulfates* / metabolism Chondroitin Sulfates* / pharmacology Mammals / metabolism Mice Muscle Fibers, Skeletal / metabolism Oligosaccharides / pharmacology Osteoclasts* / metabolism
IF 2.74
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)