| RRC ID |
74544
|
| 著者 |
Adachi O, Sugii H, Itoyama T, Fujino S, Kaneko H, Tomokiyo A, Hamano S, Hasegawa D, Obata J, Yoshida S, Kadowaki M, Sugiura R, Albougha MS, Maeda H.
|
| タイトル |
Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells.
|
| ジャーナル |
Molecules
|
| Abstract |
The aim of this study is to clarify the biological functions of decorin (DCN) in the healing and regeneration of wounded periodontal tissue. We investigated the expression pattern of DCN during the healing of wounded periodontal tissue in rats by immunohistochemistry and the effects of DCN on the osteoblastic differentiation of human periodontal ligament (PDL) stem cells (HPDLSCs) and preosteoblasts by Alizarin red S staining, quantitative reverse transcription-polymerase chain reactions, and western blotting. The expression of DCN was increased around the wounded PDL tissue on day 5 after surgery compared with the nonwounded PDL tissue, whereas its expression was not changed in the osteoblastic layer around the wounded alveolar bone. Furthermore, DCN promoted the osteoblastic differentiation of HPDLSCs, but it did not affect the osteoblastic differentiation of preosteoblasts. ERK1/2 phosphorylation was upregulated during the DCN-induced osteoblastic differentiation of HPDLSCs. DCN did not affect proliferation, migration, or the PDL-related gene expression of HPDLSCs. In conclusion, this study demonstrates that DCN has a role in the healing of wounded periodontal tissue. Furthermore, DCN secreted from PDL cells may contribute to bone healing by upregulating osteoblastic differentiation through ERK1/2 signaling in HPDLSCs, indicating a therapeutic effect of DCN in periodontal tissue regeneration.
|
| 巻・号 |
27(23)
|
| 公開日 |
2022-11-25
|
| DOI |
10.3390/molecules27238224
|
| PII |
molecules27238224
|
| PMID |
36500314
|
| PMC |
PMC9739490
|
| MeSH |
Animals
Cell Differentiation
Cell Proliferation
Cells, Cultured
Humans
Osteogenesis
Periodontal Ligament*
Rats
Signal Transduction
Stem Cells*
|
| IF |
3.267
|
| リソース情報 |
| ヒト・動物細胞 |
Saos-2(RCB0428) |
