論文 - 詳細
| RRC ID | 74561 |
|---|---|
| 著者 | Yamauchi K, Okamoto S, Ishida Y, Konno K, Hoshino K, Furuta T, Takahashi M, Koike M, Isa K, Watanabe M, Isa T, Hioki H. |
| タイトル | Fluorochromized tyramide-glucose oxidase as a multiplex fluorescent tyramide signal amplification system for histochemical analysis. |
| ジャーナル | Sci Rep |
| Abstract |
Tyramide signal amplification (TSA) is a highly sensitive method for histochemical analysis. Previously, we reported a TSA system, biotinyl tyramine-glucose oxidase (BT-GO), for bright-filed imaging. Here, we develop fluorochromized tyramide-glucose oxidase (FT-GO) as a multiplex fluorescent TSA system. FT-GO involves peroxidase-catalyzed deposition of fluorochromized tyramide (FT) with hydrogen peroxide produced by enzymatic reaction between glucose and glucose oxidase. We showed that FT-GO enhanced immunofluorescence signals while maintaining low background signals. Compared with indirect immunofluorescence detections, FT-GO demonstrated a more widespread distribution of monoaminergic projection systems in mouse and marmoset brains. For multiplex labeling with FT-GO, we quenched antibody-conjugated peroxidase using sodium azide. We applied FT-GO to multiplex fluorescent in situ hybridization, and succeeded in labeling neocortical interneuron subtypes by coupling with immunofluorescence. FT-GO immunofluorescence further increased the detectability of an adeno-associated virus tracer. Given its simplicity and a staining with a high signal-to-noise ratio, FT-GO would provide a versatile platform for histochemical analysis. |
| 巻・号 | 12(1) |
| ページ | 14807 |
| 公開日 | 2022-9-12 |
| DOI | 10.1038/s41598-022-19085-9 |
| PII | 10.1038/s41598-022-19085-9 |
| PMID | 36097273 |
| PMC | PMC9468149 |
| MeSH | Animals Coloring Agents* Fluorescent Antibody Technique Glucose Oxidase* In Situ Hybridization, Fluorescence / methods Mice Peroxidases |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |