| RRC ID |
74596
|
| Author |
Yamaguchi S, Ikeda R, Umeda Y, Kosaka T, Yamahira S, Okamoto A.
|
| Title |
Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA.
|
| Journal |
Chembiochem
|
| Abstract |
Methods to label intercellular contact have attracted attention because of their potential in cell biological and medical applications for the analysis of intercellular communications. In this study, a simple and versatile method for chemoenzymatic labeling of intercellularly contacting cells is demonstrated using a cell-surface anchoring reagent of a poly(ethylene glycol)(PEG)-lipid conjugate. The surface of each cell in the cell pairs of interest were decorated with sortase A (SrtA) and triglycine peptide that were lipidated with PEG-lipid. In the mixture of the two-cell populations, the triglycine-modified cells were enzymatically labeled with a fluorescent labeling reagent when in contact with SrtA-modified cells on a substrate. The selective labeling of the contacting cells was confirmed by confocal microscopy. The method is a promising tool for selective visualization of intercellularly contacting cells in cell mixtures for cell-cell communication analysis.
|
| Volume |
23(21)
|
| Pages |
e202200474
|
| Published |
2022-11-4
|
| DOI |
10.1002/cbic.202200474
|
| PMID |
35976800
|
| MeSH |
Aminoacyltransferases* / metabolism
Bacterial Proteins / metabolism
Cell Membrane / metabolism
Lipids
Microscopy, Confocal
Peptides / metabolism
Polyethylene Glycols / metabolism
|
| IF |
2.576
|
| Resource |
| Human and Animal Cells |
Jurkat(RCB3052) |
