RRC ID 74655
Author Schulz C.
Title Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster.
Journal Methods Mol Biol
Abstract The Notch (N) signaling pathway plays versatile roles in development and disease of many model organisms (Andersson et al., Development 138, 3593-3612, 2011; Hori et al., J Cell Sci, 126, 2135-2140, 2013). However, studying a role for N in adult tissue of Drosophila melanogaster is challenging, because the gene is located on the X-chromosome. To reduce the expression of N specifically in either the germline or the somatic cells of the adult gonad, we used the CRISPR technology in combination with the UAS/Gal4 expression system. After generation of the flies, gonads were investigated for germline survival. Here, we outline our detailed protocol.
Volume 2472
Pages 159-172
Published 2022-1-1
DOI 10.1007/978-1-0716-2201-8_13
PMID 35674899
MeSH Animals Clustered Regularly Interspaced Short Palindromic Repeats / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / metabolism Gonads / metabolism Male Receptors, Notch / genetics Receptors, Notch / metabolism Signal Transduction Technology