RRC ID 74659
Author Castañeda-Sampedro A, Calvin-Cejudo L, Martin F, Gomez-Diaz C, Alcorta E.
Title The Ntan1 gene is expressed in perineural glia and neurons of adult Drosophila.
Journal Sci Rep
Abstract The Drosophila Ntan1 gene encodes an N-terminal asparagine amidohydrolase that we show is highly conserved throughout evolution. Protein isoforms share more than 72% of similarity with their human counterparts. At the cellular level, this gene regulates the type of glial cell growth in Drosophila larvae by its different expression levels. The Drosophila Ntan1 gene has 4 transcripts that encode 2 protein isoforms. Here we describe that although this gene is expressed at all developmental stages and adult organs tested (eye, antennae and brain) there are some transcript-dependent specificities. Therefore, both quantitative and qualitative cues could account for gene function. However, widespread developmental stage and organ-dependent expression could be masking cell-specific constraints that can be explored in Drosophila by using Gal4 drivers. We report a new genetic driver within this gene, Mz317-Gal4, that recapitulates the Ntan1 gene expression pattern in adults. It shows specific expression for perineural glia in the olfactory organs but mixed expression with some neurons in the adult brain. Memory and social behavior disturbances in mice and cancer and schizophrenia in humans have been linked to the Ntan1 gene. Therefore, these new tools in Drosophila may contribute to our understanding of the cellular basis of these alterations.
Volume 12(1)
Pages 14749
Published 2022-8-30
DOI 10.1038/s41598-022-18999-8
PII 10.1038/s41598-022-18999-8
PMID 36042338
PMC PMC9427837
MeSH Animals Drosophila* / genetics Drosophila* / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Humans Mice Neuroglia / metabolism Neurons / metabolism Phenotype
IF 3.998
Drosophila DGRC#103887