| RRC ID |
74667
|
| 著者 |
Kinoshita S, Takarada K, Kinoshita Y, Inoue YH.
|
| タイトル |
Drosophila hemocytes recognize lymph gland tumors of mxc mutants and activate the innate immune pathway in a reactive oxygen species-dependent manner.
|
| ジャーナル |
Biol Open
|
| Abstract |
Mechanisms of cancer cell recognition and elimination by the innate immune system remains unclear. The immune signaling pathways are activated in the fat body to suppress the tumor growth in mxcmbn1 hematopoietic tumor mutants in Drosophila by inducing antimicrobial peptides (AMP). Here, we investigated the regulatory mechanism underlying the activation in the mutant. Firstly, we found that reactive oxygen species (ROS) accumulated in the hemocytes due to induction of dual oxidase and one of its activators. This was required for the AMP induction and the tumor growth suppression. Next, more hemocytes transplanted from normal larvae were associated with the mutant tumor than normal lymph glands (LGs). Matrix metalloproteinase 1 and 2 (MMP2) were highly expressed in the tumors. The basement membrane components in the tumors were reduced and ultimately lost inside. Depletion of the MMP2 rather than MMP1 resulted in a significantly reduced AMP expression in the mutant larvae. The hemocytes may recognize the disassembly of basement membrane in the tumors and activate the ROS production. Our findings highlight the mechanism via which macrophage-like hemocytes recognize tumor cells and subsequently convey the information to induce AMPs in the fat body. They contribute to uncover the role of innate immune system against cancer.
|
| 巻・号 |
11(11)
|
| 公開日 |
2022-11-1
|
| DOI |
10.1242/bio.059523
|
| PII |
277211
|
| PMID |
36226812
|
| PMC |
PMC9641529
|
| MeSH |
Animals
Drosophila* / metabolism
Drosophila Proteins* / genetics
Drosophila Proteins* / metabolism
Hemocytes / metabolism
Immunity, Innate
Larva / metabolism
Matrix Metalloproteinase 2 / metabolism
Reactive Oxygen Species / metabolism
Tumor Suppressor Proteins
|
| IF |
2.029
|
| リソース情報 |
| ショウジョウバエ |
DGRC#103625 |
