RRC ID 74729
Author Yuen AC, Prasad AR, Fernandes VM, Amoyel M.
Title A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila.
Journal Biol Open
Abstract Extracellular signal-regulated kinase (ERK) lies downstream of a core signalling cascade that controls all aspects of development and adult homeostasis. Recent developments have led to new tools to image and manipulate the pathway. However, visualising ERK activity in vivo with high temporal resolution remains a challenge in Drosophila. We adapted a kinase translocation reporter (KTR) for use in Drosophila, which shuttles out of the nucleus when phosphorylated by ERK. We show that ERK-KTR faithfully reports endogenous ERK signalling activity in developing and adult tissues, and that it responds to genetic perturbations upstream of ERK. Using ERK-KTR in time-lapse imaging, we made two novel observations: firstly, sustained hyperactivation of ERK by expression of dominant-active epidermal growth factor receptor raised the overall level but did not alter the kinetics of ERK activity; secondly, the direction of migration of retinal basal glia correlated with their ERK activity levels, suggesting an explanation for the heterogeneity in ERK activity observed in fixed tissue. Our results show that KTR technology can be applied in Drosophila to monitor ERK activity in real-time and suggest that this modular tool can be further adapted to study other kinases. This article has an associated First Person interview with the first author of the paper.
Volume 11(5)
Published 2022-5-15
DOI 10.1242/bio.059364
PII 275453
PMID 35608229
PMC PMC9167624
MeSH Animals Drosophila* / genetics Drosophila* / metabolism Extracellular Signal-Regulated MAP Kinases / metabolism MAP Kinase Signaling System* Phosphorylation Signal Transduction
IF 2.029
Drosophila DGRC#104055