論文 - 詳細
| RRC ID | 74750 |
|---|---|
| 著者 | Dark C, Cheung S, Cheng LY. |
| タイトル | Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae. |
| ジャーナル | STAR Protoc |
| Abstract |
Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021). |
| 巻・号 | 3(1) |
| ページ | 101230 |
| 公開日 | 2022-3-18 |
| DOI | 10.1016/j.xpro.2022.101230 |
| PII | S2666-1667(22)00110-1 |
| PMID | 35284841 |
| PMC | PMC8915010 |
| MeSH | Animals Cachexia* / metabolism Drosophila* Fat Body / metabolism Larva / metabolism Muscles / metabolism Phenotype |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#110692 DGRC#115262 |