論文 - 詳細
| RRC ID | 74751 |
|---|---|
| 著者 | Balaraju AK, Hu B, Rodriguez JJ, Murry M, Lin F. |
| タイトル | Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2. |
| ジャーナル | Development |
| Abstract |
Noncanonical Wnt/planar cell polarity (Wnt/PCP) signaling has been implicated in endoderm morphogenesis. However, the underlying cellular and molecular mechanisms of this process are unclear. We found that, during convergence and extension (C&E) in zebrafish, gut endodermal cells are polarized mediolaterally, with GFP-Vangl2 enriched at the anterior edges. Endoderm cell polarity is lost and intercalation is impaired in the absence of glypican 4 (gpc4), a heparan-sulfate proteoglycan that promotes Wnt/PCP signaling, suggesting that this signaling is required for endodermal cell polarity. Live imaging revealed that endoderm C&E is accomplished by polarized cell protrusions and junction remodeling, which are impaired in gpc4-deficient endodermal cells. Furthermore, in the absence of gpc4, Cadherin 2 expression on the endodermal cell surface is increased as a result of impaired Rab5c-mediated endocytosis, which partially accounts for the endodermal defects in these mutants. These findings indicate that Gpc4 regulates endodermal planar cell polarity during endoderm C&E by influencing the localization of Cadherin 2. Thus, our study uncovers a new mechanism by which Gpc4 regulates planar cell polarity and reveals the role of Wnt/PCP signaling in endoderm morphogenesis. |
| 巻・号 | 148(14) |
| 公開日 | 2021-7-15 |
| DOI | 10.1242/dev.199421 |
| PII | 270869 |
| PMID | 34131730 |
| PMC | PMC8313861 |
| MeSH | Animals Cadherins / metabolism* Cell Polarity / physiology* Endoderm / metabolism* Gastrulation Gene Expression Regulation, Developmental Glypicans / metabolism* Heparan Sulfate Proteoglycans / metabolism Morphogenesis Wnt Proteins / metabolism Wnt Signaling Pathway Zebrafish / metabolism Zebrafish Proteins / metabolism* rab5 GTP-Binding Proteins |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(sox17:EGFP) |