RRC ID 74843
Author Nishiguchi A, Taguchi T.
Title Designing an anti-inflammatory and tissue-adhesive colloidal dressing for wound treatment.
Journal Colloids Surf B Biointerfaces
Abstract Wound dressing materials are widely used to protect wounds from the external environment and to promote wound healing. However, conventional wound dressings lack tissue adhesive properties and anti-inflammatory functions, which lead to fibrosis and stricture, in cases such as gastrointestinal wounds after endoscopic surgery. In the current study, we report tissue-adhesive and anti-inflammatory properties of a wound dressing composed of corticosteroid-modified gelatin particles. Hydrocortisone (HC), which is a class of anti-inflammatory corticosteroid, was used to modify Alaska-pollock gelatin (ApGltn) to synthesize HC-modified ApGltn (HC-ApGltn). Microparticles (MPs) of HC-ApGltn were fabricated by adding ethanol in HC-ApGltn aqueous solution and performing thermal crosslinking (TC) without the use of toxic surfactants and crosslinking reagents. Modification of ApGltn with hydrophobic HC containing cholesterol backbone structure improved its adhesion strength to gastric submucosal tissues under wet conditions owing to hydrophobic interactions. This retention of adhesive property under wet conditions allows for stable protection of wounds from the external environment. We found that HC-ApGltn MPs were taken up by macrophages and they effectively suppressed morphological changes of LPS-activated macrophages and the expression level of the inflammatory cytokine. Robust tissue adhesive and anti-inflammatory MPs may serve as an advanced wound dressing that can protect wounds and suppress inflammatory responses for promoting wound healing.
Volume 188
Pages 110737
Published 2020-4-1
DOI 10.1016/j.colsurfb.2019.110737
PII S0927-7765(19)30881-1
PMID 32004904
MeSH Animals Anti-Inflammatory Agents / chemical synthesis Anti-Inflammatory Agents / chemistry Anti-Inflammatory Agents / pharmacology* Cell Line Drug Design* Gelatin / chemical synthesis Gelatin / chemistry Gelatin / pharmacology* Hydrocortisone / chemical synthesis Hydrocortisone / chemistry Hydrocortisone / pharmacology* Mice Molecular Conformation Particle Size Surface Properties Tissue Adhesives / chemical synthesis Tissue Adhesives / chemistry Tissue Adhesives / pharmacology* Wound Healing / drug effects*
IF 4.389
Human and Animal Cells RAW 264(RCB0535)